|
|
Blood, 1 August 2006, Vol. 108, No. 3, pp. 965-973.
Prepublished online as a Blood First Edition Paper on April 6, 2006; DOI 10.1182/blood-2005-12-5046.
 Previous Article | Next Article 
Submitted December 21, 2005
Accepted March 21, 2006
The Wingless homologue Wnt5a and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation
Antje Blumenthal, Stefan Ehlers, Jorg Lauber, Jan Buer, Christoph Lange, Torsten Goldmann, Holger Heine, Ernst Brandt, and Norbert Reiling*
Division of Molecular Infection Biology, Research Center Borstel (RCB), Borstel, Germany
Department of Mucosal Immunity, The German Research Center for Biotechnology (GBF), Braunschweig, Germany
Department of Mucosal Immunity, The German Research Center for Biotechnology (GBF), Braunschweig, Germany; Institute of Medical Microbiology, Hannover Medical School, Hannover, Germany
Division of Clinical Infectious Diseases, Research Center Borstel, Borstel, Germany
Division of Clinical and Experimental Pathology, Research Center Borstel, Borstel, Germany
Divison of Innate Immunity, Research Center Borstel, Borstel, Germany
Division of Biological Chemistry, Research Center Borstel, Borstel, Germany
* Corresponding author; email: nreiling{at}fz-borstel.de.
Microarray-assisted gene expression screens of human macrophages revealed Wnt5a, a homologue of Wingless, a key regulator of Drosophila melanogaster embryonic segmentation and patterning, to be consistently upregulated following stimulation with different mycobacterial species and conserved bacterial structures. The expression of Wnt5a required TLR signaling and NF- B activation, which identifies a novel induction pathway for a Wingless homologue. We show that human peripheral blood mononuclear cells express the Wnt5a receptor Frizzled-5 (Fzd5). Both, Wnt5a and Fzd5, were also detected in granulomatous lesions in the lungs of M. tuberculosis infected patients. Functional studies showed that Wnt5a and Fzd5 regulate the microbially induced IL-12 response of antigen presenting cells and IFN- production by mycobacterial antigen-stimulated T cells. Our findings implicate the evolutionarily conserved Wnt/Frizzled signaling system in bridging innate and adaptive immunity to infections.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
Related Article in Blood Online:
-
Macrophage activation:'tis the way the WNT blows
- Jeff Schorey
Blood 2006 108: 784.
[Full Text]
[PDF]
This article has been cited by other articles:
|
|
|
|
 
T. Ueland, K. Otterdal, T. Lekva, B. Halvorsen, A. Gabrielsen, W. J. Sandberg, G. Paulsson-Berne, T. M. Pedersen, L. Folkersen, L. Gullestad, et al.
Dickkopf-1 Enhances Inflammatory Interaction Between Platelets and Endothelial Cells and Shows Increased Expression in Atherosclerosis
Arterioscler Thromb Vasc Biol,
August 1, 2009;
29(8):
1228 - 1234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Entschladen, J. A. Lindquist, E. Serfling, G. Thiel, A. Kieser, K. Giehl, C. Ehrhardt, S. M. Feller, O. Ullrich, F. Schaper, et al.
Signal Transduction--Receptors, Mediators, and Genes
Sci. Signal.,
March 24, 2009;
2(63):
mr3 - mr3.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Sen and G. Ghosh
Transcriptional Outcome of Wnt-Frizzled Signal Transduction in Inflammation: Evolving Concepts
J. Immunol.,
October 1, 2008;
181(7):
4441 - 4445.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. I. Pacheco and R. J. MacLeod
CaSR stimulates secretion of Wnt5a from colonic myofibroblasts to stimulate CDX2 and sucrase-isomaltase using Ror2 on intestinal epithelia
Am J Physiol Gastrointest Liver Physiol,
October 1, 2008;
295(4):
G748 - G759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Christman II, D. J. Goetz, E. Dickerson, K. D. McCall, C. J. Lewis, F. Benencia, M. J. Silver, L. D. Kohn, and R. Malgor
Wnt5a is expressed in murine and human atherosclerotic lesions
Am J Physiol Heart Circ Physiol,
June 1, 2008;
294(6):
H2864 - H2870.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. George
Wnt Pathway: A New Role in Regulation of Inflammation
Arterioscler Thromb Vasc Biol,
March 1, 2008;
28(3):
400 - 402.
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Pereira, D. J. Schaer, E. B. Bachli, M. O. Kurrer, and G. Schoedon
Wnt5A/CaMKII Signaling Contributes to the Inflammatory Response of Macrophages and Is a Target for the Antiinflammatory Action of Activated Protein C and Interleukin-10
Arterioscler Thromb Vasc Biol,
March 1, 2008;
28(3):
504 - 510.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Lehtonen, H. Ahlfors, V. Veckman, M. Miettinen, R. Lahesmaa, and I. Julkunen
Gene expression profiling during differentiation of human monocytes to macrophages or dendritic cells
J. Leukoc. Biol.,
September 1, 2007;
82(3):
710 - 720.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. McCall, N. Harii, C. J. Lewis, R. Malgor, W. Bae Kim, M. Saji, A. D. Kohn, R. T. Moon, and L. D. Kohn
High Basal Levels of Functional Toll-Like Receptor 3 (TLR3) and Noncanonical Wnt5a Are Expressed in Papillary Thyroid Cancer and Are Coordinately Decreased by Phenylmethimazole Together with Cell Proliferation and Migration
Endocrinology,
September 1, 2007;
148(9):
4226 - 4237.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. MacLeod, M. Hayes, and I. Pacheco
Wnt5a secretion stimulated by the extracellular calcium-sensing receptor inhibits defective Wnt signaling in colon cancer cells
Am J Physiol Gastrointest Liver Physiol,
July 1, 2007;
293(1):
G403 - G411.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
