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Blood, 15 June 2006, Vol. 107, No. 12, pp. 4711-4713. Prepublished online as a Blood First Edition Paper on February 21, 2006; DOI 10.1182/blood-2006-01-0028. This Article Full Text (PDF) All Versions of this Article: 2006-01-0028v1 107/12/4711    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Beullens, M. Articles by Bollen, M. Search for Related Content PubMed PubMed Citation Articles by Beullens, M. Articles by Bollen, M. Related Collections Related Letters in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 5, 2006 Accepted February 11, 2006 False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise Monique Beullens, Joris R Delanghe, and Mathieu Bollen* Division of Biochemistry, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University of Leuven, Leuven, Belgium Department of Clinical Chemistry, University Hospital, Gent, Belgium * Corresponding author; email: Mathieu.Bollen{at}med.kuleuven.be. Erythropoietin (Epo) is a glycoprotein hormone that promotes the production of red blood cells. Recombinant human Epo (rhEpo) is illicitly used to improve performance in endurance sports. Doping in sports is discouraged by the screening of athletes for rhEPO in urine. The adopted test is based on a combination of isoelectric focusing and double immunoblotting, and distinguishes between endogenous and recombinant human Epo. We show here that this widely-used test can occasionally lead to the false-positive detection of rhEpo (epoetin-) in post-exercise, protein-rich urine, probably because the adopted monoclonal anti-Epo antibodies are not monospecific. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letters in Blood Online: False-positive Epo test concerns unfounded Don Catlin, Gary Green, Michael Sekera, Paul Scott, and Borislav Starcevic Blood 2006 108: 1778. [Full Text] [PDF] No doubt about the validity of the urine test for detection of recombinant human erythropoietin Françoise Lasne, Monique Beullens, Joris R. Delanghe, and Mathieu Bollen Blood 2006 108: 1778-1780. [Full Text] [PDF] This article has been cited by other articles: C. Lundby, N. J. Achman-Andersen, J. J. Thomsen, A. M. Norgaard, and P. Robach Testing for recombinant human erythropoietin in urine: problems associated with current anti-doping testing J Appl Physiol, August 1, 2008; 105(2): 417 - 419. [Abstract] [Full Text] [PDF] D. Catlin, G. Green, M. Sekera, P. Scott, and B. Starcevic False-positive Epo test concerns unfounded. Blood, September 1, 2006; 108(5): 1778 - 1778. [Full Text] [PDF] F. Lasne, M. Beullens, J. R. Delanghe, and M. Bollen No doubt about the validity of the urine test for detection of recombinant human erythropoietin. Blood, September 1, 2006; 108(5): 1778 - 1780. [Full Text] [PDF]

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