|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
CP Burns
The metabolism of an essential amino acid, isoleucine, by human leukemic
and gradient-separated normal human leukocytes of various types and
maturity was studied. Blood leukocytes were isolated and incubated with
(U-14C) isoleucine. Separation of metabolic intermediates was accomplished
by sequential extraction. The rate of isoleucine incorporation into protein
by immature cells from untreated patients with acute leukemia (15.9 plus or
minus 2.4 nmoles/hr per 10-8 leukocytes) was considerably higher than the
rates of incorporation by mature neutrophils (3.2 plus or minus 0.5
nmoles/hr per 10-8 leukocytes), lymphocytes (7.7 plus or minus 1.2
nmoles/hr per 10-8 leukocytes), and eosinophils (6.2 plus or minus 1.3
nmoles/hr per 10-8 leukocytes). Those cell preparations with more blast
cells had higher rates of protein synthesis. In addition, those cells with
greater thymidine incorporation had higher rates of protein synthesis. The
leukocytes both oxidized isoleucine and incorporated it into cell
isoleucine and incorporated it into cell lipid. The rates of these
metabolic processes were characteristic for various types and maturity of
leukocytes. This study demonstrates a relationship of rate of protein
synthesis to leukocyte immaturity. This relationship is maintained in
neoplastic leukocytes. It suggests that the requirement of the mitotic
process for newly synthesized protein is greater than that for the
elaboration of the protein products of the mature leukocyte.
This article has been cited by other articles:
| |||||||||||
 |
 |
 |
|||||||
 |
 |
 |
 |
 |
 |
 |
 |
||
| Copyright © 1975 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
