Modulation of murine granulocyte proliferation in diffusion chamber
cultures
JB Marmor, JL Russell, AM Miller and SH Robinson
Normal mouse marrow cells were cultured in Millipore diffusion chambers for
long periods of time and at a variety of cell concentrations. All cultures
showed a pattern of granulocyte proliferation characterized by logarithmic
growth followed by prolonged stabilization of cell number starting a 7 days
thereafter. The height of this "plateau" varied in relationship to the
level of cell input and characteristically was far lower than the maximum
cell density that can be maintained in this culture system. Additional
studies showed that the plateau represented a steady state of granulocyte
turnover and was not due to alterations in the diffusion chambers or the
host mice. Regulatory mechanisms intrinsic to the cultured cell population
appeared to play a primary role in maintaining this stable plateau.
Modulation of granulocyte proliferation was partly due to increasing cell
density; particularly with high input concentrations. In addition,
differential cell counts suggested that critical changes in the
relationship between immature and mature granulocytes partially accounted
for this apparent autoregulation of cell growth. The plateau period in
diffusion chamber cultures in many ways resembles granulocyte proliferation
in normal mouse bone marrow and is a useful model for the study of
regulatory functions in granulocytopoiesis.
Volume 46,
Issue 1,
pp. 39-50,
07/01/1975
Copyright © 1975 by The American Society of Hematology
