Effect of papain on the interaction between human monocytes, erythrocytes,
and IgG
MA Kenna, RA Cooper and AD Schrieber
The mechanism by which papain detaches IgG-sensitized erythrocytes from the
monocyte surface has been explored in an in vitro assay for the monocyte
IgG receptor using red cells quantitatively sensitized with IgG anti-Rh D
immunoglobulin. Papain treatment of IgG-sensitized erythrocytes diminished
the ability of these cells to bind to the monocyte surface; however,
treatment of erythrocytes with papain prior to sensitization with IgG did
not inhibit binding, and at papain concentrations is greater than or equal
to 38 mug/ml binding was enhanced. IgG receptor activity was not diminished
by prior treatment of monolayer cells with papain and was enhanced with
high concentrations of papain. These studies suggest that papain detaches
erythrocytes from the monocyte surface by virtue of its proteolytic effect
on IgG and not by an effect of papain on the D antigen of red cells or the
IgG receptor on monocytes.
Volume 46,
Issue 2,
pp. 245-252,
08/01/1975
Copyright © 1975 by The American Society of Hematology
