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JF Di Persio, JK Brennan, MA Lichtman and BL Speiser
We have established two human cell lines which elaborate colony-
stimulating activity (CSA) for at least four species: man, mouse, rabbit,
and dog. One, GCT, was isolated from a lung metastasis of a fibrous
histiocytoma; the other, RC4, from a monocyte-enriched fraction of normal
blood. Medium conditioned by either GCT or RC4 cells was more potent in
stimulating human marrow growth in vitro than was monocyte- conditioned
medium or human leukocyte feeder layers. Fractionation of
cell-line-conditioned medium by Sephacryl S-200 chromatography indicated
that the maximum activity of the CSA for human marrow cells is eluted
within the range of 30,000-40,000 daltons. These cells lines provide a
continuous source of large quantities of conditioned medium for
purification of CSA. Moreover, the invariable growth-supporting activity
for all species tested and the high potency of cell-line CSA facilitates
studies of its elaboration and biologic effects.
This article has been cited by other articles:
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| Copyright © 1978 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
