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Cation permeability alterations during sickling: relationship to cation
composition and cellular hydration of irreversibly sickled cells
BE Glader and DG Nathan
Sickle erythrocytes (RBC) incubated under 100% nitrogen for 4 hr manifested
marked Na gain with an equivalent K loss. There were no changes in cell
total cation or water content under these conditions, and no irreversible
sickle cells (ISC) were formed. In contrast, sickle RBC incubated for 24 hr
under 100% nitrogen in a glucose-free Na medium containing calcium
manifested marked ISC formation. ISC formed under these conditions also had
elevated Na content, although K content was much more reduced, and
consequently ISC were cation depleted and dehydrated. When sickle RBC were
incubated 24 hr under 100% nitrogen in a glucose-free K medium containing
calcium no ISC formed and there were no major changes in cation or water
content. These studies indicate that the Na+K content and dehydration of
ISC was not directly related to the increased cation permeability
associated with sickling. Rather, the ISC changes appear to reflect the
well-known Gardos effect (K and water loss occurring in ATP-depleted RBC
incubated with calcium). In addition, these studies suggest that ISC
formation per se is related to K and water loss, since no ISC were formed
when ATP-depleted sickle RBC were deoxygenated in calcium-containing high-K
media that prevented K loss and dehydration.
Volume 51,
Issue 5,
pp. 983-989,
05/01/1978
Copyright © 1978 by The American Society of Hematology
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