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An assay for intrinsic factor based on blocking of the R binder of gastric juice by cobinamide

JA Begley and A Trachtenberg

An in vitro assay for measurement of gastric juice intrinsic factor (IF) was developed based on the ability of the cobinamide (Cbi) [(CN, OH) Cbi] to bind to the gastric juice R-type binders of cobalamin (Cbl) and not to the IF binder. Subsequently added radioactive Cbl, CN-[57Co] Cbl, binds only to the IF binders and allows for direct measurement of this Cbl binding protein. This Cbi blocking assay was found to function as well as the more conventional methods of IF measurement, G-100 column chromatography, and IF blocking antibody assay. The present assay has the advantage of eliminating the need for elaborate forms of protein separation and does not rely on a source of antibody.

Volume 53, Issue 4, pp. 788-793, 04/01/1979
Copyright © 1979 by The American Society of Hematology


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  Copyright © 1979 by American Society of Hematology         Online ISSN: 1528-0020