Membrane receptors and their redistribution in lymphoproliferative
disorders
F Naeim, K Bergmann and RA Gatti
Lymphoid cells from 20 patients with lymphoproliferative disorders,
including chronic lymphocytic leukemia, hairy cell leukemia, Sezary
syndrome, lymphoma, and lymphadenitis, were studied for redistribution of
surface membrane immunoglobulins (SmIg) and concanavalin A (Con-A)
receptors. Fluorescein-labeled polyvalent goat anti-human immunoglobulin
and fluoresceinated concanavalin A were used as ligands. Results were
similar with both ligands. The highest percentage of capping of
ligand-membrane receptors was noted in mononuclear cells from patients with
"hairy" cell leukemia: from 24% to 90%. These cells showed moderate to
marked fluorescein activity and were able to cap within 15 min at 4 degrees
C. Chronic lymphocytic leukemia cells showed a weak fluorescein stain with
a very low percentage of cells (0%--16%) capping. Lymph node cells from
patients with lymphoma demonstrated moderate to strong fluorescein activity
with only an average of 3% of the cells capping; while lymphoid cells from
patients with lymphaedenitis showed an average of 27.5% capping and
moderate fluorescein activity. Capping of Con-A receptors in mononuclear
cells from patients with Sezary syndrome was poor (0%--14%) with moderate
fluorescein intensity. This report demonstrates difference in density and
mobility of binding sites for SmIg and Con-A on the surface membrane of
lymphoid cells from various subclasses of lymphoproliferative disorders.
These differences may assist in the differential diagnosis and
classification of these conditions.
Volume 54,
Issue 3,
pp. 648-658,
09/01/1979
Copyright © 1979 by The American Society of Hematology
