Differential regulation of spleen cell-mediated eosinophil and
neutrophil-macrophage production
SH Bartelmez, WH Dodge and DA Bass
Nonadherent spleen cells of mice infected with Trichinella spiralis
released growth stimulatory factors (GSFs) in vitro when challenged with
excretory/secretory products of muscle stage larvae. The assay of GSF was
based on proliferation of normal, nonadherent syngeneic marrow cells in
liquid tube cultures. Media conditioned for 1 day by challenged spleen
cells stimulated eosinophil production but failed to stimulate production
of other cell types. In contrast, media conditioned for 5 days supported
eosinophil, neutrophil, and macrophage production. The kinetics of cell
production were also different. Eosinophil production started within 1 day,
reached a peak at day 2, and was down to control levels by day 4. In
contrast, neutrophil/macrophage production began between 2 and 4 days and
reached a peak at 6--8 days. The short duration of eosinophil production
was evidently due to depletion of growth-factor-responsive cells.
Volume 55,
Issue 3,
pp. 489-493,
03/01/1980
Copyright © 1980 by The American Society of Hematology
