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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Steinberg, S. E. Articles by Hillman, R. S. Search for Related Content PubMed PubMed Citation Articles by Steinberg, S. E. Articles by Hillman, R. S. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  The effect of trimethoprim/sulfamethoxazole on Friend erythroleukemia cells SE Steinberg, CL Campbell, PS Rabinovitch and RS Hillman Cultures of Friend erythroleukemia cells were subjected to the antibiotics trimethoprim (T) and sulfamethoxazole (S) at levels equal to or below the usual therapeutic range. At T 8 microgram/ml and S 40 microgram/ml, cell growth was arrested, cells appeared megaloblastic, and the examination of cell-cycle distribution by flow microfluorimetry revealed arrest in S phase. With a tenfold reduction in drug levels (T, 08 microgram/ml; S, 4 microgram/ml) cell growth was less markedly inhibited, morphology remained megaloblastic, and S-phase block was still dramatic. A further tenfold reduction (T, 0.08 microgram/ml; S, 0.4 microgram/ml), well below effective antibacterial levels, allowed normal cell growth and morphology but DNA synthesis was still inhibited. Additions of folinic acid at 100 ng/ml averted all drug effects. Thus T/S can affect cell replication even at levels well below those usually employed and could prolong the rate of recovery of hematopoietic cells in the myelosuppressed patient. Volume 55, Issue 3, pp. 501-504, 03/01/1980 Copyright © 1980 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. P. BRADLEY, G. D. WARDEN, J. G. MAXWELL, and G. ROTHSTEIN Neutropenia and Thrombocytopenia in Renal Allograft Recipients Treated with Trimethoprim-Sulfamethoxazole Ann Intern Med, October 1, 1980; 93(4): 560 - 562. [Abstract] [PDF]

	Copyright © 1980 by American Society of Hematology         Online ISSN: 1528-0020