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The mechanism of thrombin-induced platelet factor 4 secretion
MH Ginsberg, L Taylor and RG Painter
We have measured thrombin-induced secretion of platelet factor 4 antigen
(PF4) and simultaneously followed its intracellular translocation by
immunofluorescence. In permeable resting platelets, speckled intracellular
immunofluorescent staining for PF4 was observed. Addition of thrombin to
washed platelets at 22 degrees C resulted in secretion of PF4 and formation
of large (approximately 0.5 micrometer) immunofluorescent masses. These
masses moved to the cell periphery during secretion and were virtually
absent at the conclusion of secretion. Ultrastructural examination of
thrombin-treated platelets revealed vacuoles corresponding in size, shape,
and time of occurrence to the large immunofluorescent masses of PF4. These
vacuoles contained PF4 by immunoferritin staining of frozen thin sections;
they therefore appear to represent the ultrastructural counterpart of the
large PF4 masses. When intact cells were stained for PF4 after thrombin
addition, only 5.6% of the large masses stained. Thus, during secretion,
PF4 antigen is consolidated into large closed pools that appear as vacuoles
in the electron microscope.
Volume 55,
Issue 4,
pp. 661-668,
04/01/1980
Copyright © 1980 by The American Society of Hematology
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