Purification of hemopoietic progenitor cells from human marrow using a
fucose-binding lectin and cell sorting
G Morstyn, NA Nicola and D Metcalf
Human peripheral blood granulocytes, but not lymphocytes, erythrocytes, or
monocytes, bound the fucose-binding lectin from Lotus tetragonolobus (FBP),
and this binding was competitively inhibited by the sugar alpha- L-fucose.
The fluorescence-activated cell sorter was used to study the appearance of
this receptor on human marrow cells during granulocyte differentiation and
to prepare fractions enriched for granulocyte- macrophage progenitor cells
(granulocyte-macrophage colony-forming cells--GM-CFC). Cell binding of
fluoresceinated FBP increased for bone marrow cells in the
sequence--lymphocytes, blast cells, promyelocytes and myelocytes,
monocytes, and polymorphonuclear cells. Selection of cells with appropriate
low-angle or high-angle light scatter characteristics achieved a 10-fold or
2-3-fold enrichment of progenitor cells, respectively. By selecting cells
with intermediate fluorescence intensity, a further 2-3-fold enrichment for
GM-CFC was obtained. Cell sorting using the optimal selection of these
three parameters produced up to 36-fold enrichment of the progenitor cells
from human bone marrow. The most enriched fraction was composed of 23%
progenitor cells (colony- and cluster-forming cells) with a yield of 36%.
In populations most highly enriched by GM-CFC, immature cells (blast cells,
promyelocytes, and myelocytes) made up 95% of the cells present.
Volume 56,
Issue 5,
pp. 798-805,
11/01/1980
Copyright © 1980 by The American Society of Hematology
