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M Bar-Eli, MC Territo and MJ Cline
Alveolar and peritoneal macrophages differ in their energy metabolism.
Alveolar macrophages are mainly aerobic whereas peritoneal macrophages are
mainly anaerobic in their energy generation. We investigated the question
of whether these differences in metabolism are preprogrammed in subsets of
macrophage precursors in the bone marrow, or develop in proliferating cells
as a consequence of exposure to different tissue environments. The progeny
of single mouse macrophage progenitor cells were grown in vitro for 4 days;
the resultant colonies were divided into two roughly equal populations,
which were cultured in either a high or low oxygen environment
corresponding to that of the alveoli or tissues. Following 4 days
incubation at 5% or 20% O2, the activities of the two glycolytic enzymes
lactate dehydrogenase (LDH) and pyruvate kinase (PK) were two- to threefold
higher in the half of the colonies grown in the low O2 environment, whereas
the activity of the oxidative phosphorylative enzyme glutamate
dehydrogenase (GDH) was two- to threefold higher in the half colony grown
in the aerobic environment. Re-exposure of the cells from the low O2
environment to high O2 conditions for an additional 4 days caused a rise in
the GDH activity and a decrease in the LDH and PK. The recovery of the GDH
activity after the re-exposure was time dependent. Our results support the
theory that macrophages arising from a single progenitor cell can develop
different metabolic features depending on the O2 environment in which they
mature. A single precursor cell can give rise to mature cells with
metabolic characteristic of either alveolar or tissue macrophages.
This article has been cited by other articles:
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| Copyright © 1981 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
