A new system of hemopoietic colony formation for permanent slides and
medium changes: use of glass-fiber filters
F Kodama, A Maruta, S Motomura, K Fukushima and M Umeda
A new method for hemopoietic colony formation that allows the preparation
of permanent slides and medium changes during the incubation period was
developed in vitro. Bone marrow cells from mice were spread over
glass-fiber filters, which were placed on agar medium and cultivated for 7
days. Hemopoietic colonies appeared on the glass- fibers filters. The
glass-fiber filters with colonies were stained by a peroxidase and
nonspecific esterase double-staining method and mounted as permanent
slides. Each colony could be clearly identified and easily counted after
the staining. The dose-response relationship between the number of seeded
cells and the colony counts was a linear one, with the line very nearly
passing through the origin on extrapolation. The colonies were classified
into three types by the staining results: granuloid type,
monocyte-macrophage type, and mixed type, the last containing both
granuloid and monocyte-macrophage cells. Medium change during the
incubation period was attempted in the experiment for phagocytic activity
of the cultured cells and proved to be useful. This system appears to be
useful and convenient in the study of hemopoietic colony formation.
Volume 57,
Issue 6,
pp. 1119-1124,
06/01/1981
Copyright © 1981 by The American Society of Hematology
