Defective neutrophil migration in monosomy-7
P Ruutu, T Ruutu, H Repo, P Vuopio, T Timonen, TU Kosunen and A de la Chapelle
The migration in vitro of neutrophils from six patients with monosomy-7 or
partial deletion of the long arm of chromosome 7 was studied by two
methods: the Millipore filter assay and the migration under agarose assay.
Four of the patients had preleukemia, one had subacute myelomonocytic
leukemia, and one polycythemia vera. In four patients, chemotaxis
(migration towards a higher concentration of chemoattractant) and
chemokinesis (stimulated migration without a gradient) were shown to be
defective by both methods. In the remaining two patients, this defect could
be demonstrated only by the Millipore filter assay or by the agarose assay.
Under agarose, random locomotion (no chemoattractant present) of the
patients' neutrophils was less than that of the control subjects in four
patients, whereas no clear difference could be shown by the Millipore
filter method. This study demonstrates that the previously described defect
of neutrophil migration in monosomy-7 involves not only chemotaxis but all
stimulated migration and, at least in some patients, random locomotion as
well. Defective migration in two patients with an apparently terminal
deletion of the long arm of one chromosome 7 indicates that the distal half
of 7q carries genetic material important for neutrophil locomotion.
Volume 58,
Issue 4,
pp. 739-745,
10/01/1981
Copyright © 1981 by The American Society of Hematology
