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Immature Megakaryocytes in the Mouse: Morphology and quantitation by
acetylcholinesterase staining
MW Long and N Williams
Three types of immature megakaryocytes, detected by their morphological
properties, have been characterized in bone marrow of normal C57BL/6 mice.
Morphological classification of these cells was carried out by determining
(1) presence and relative amount of acetylcholinesterase, (2) cell size,
(3) nuclear/cytoplasm ratio, and (4) nuclear shape. The immature
megakaryocytes were classified as: (A) cells distinguished by a round
nucleus (10.6 +/- 1.1 mu diameter; mean +/- SEM), which had the highest
nucleus / cytoplasm ratio and lowest content of acetylcholinesterase; (B)
cells with an indented nucleus (13.0 +/- 1.9 mu diameter), which had
increased acetylcholinesterase content and reduced nucleus/cytoplasm ratio
compared to the round-nucleus cell type; and (C) lobed-nucleus cells (14.5
+/- 2.9 mu diameter), which showed further increase in acetylcholinesterase
content and reduction in nucleus/cytoplasm ratio. Increased numbers of
immature megakaryocytes were detected, indicating that a proportion of
these cells are undetected using conventional staining techniques. Based on
the observed alterations in size, acetylcholinesterase content, and nuclear
complexity, it was concluded that these cells constitute part of a
progressive maturation sequence intermediate between the progenitor cell
(CFU-Mk) and mature easily recognizable megakaryocytes.
Volume 58,
Issue 5,
pp. 1032-1039,
11/01/1981
Copyright © 1981 by The American Society of Hematology
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