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Opsonized zymosan-stimulated granulocytes-activation and activity of the
superoxide-generating system and membrane potential changes
HJ Cohen, PE Newburger, ME Chovaniec, JC Whitin and ER Simons
Phagocytic cells generate superoxide in response to stimulation by
opsonized particles. A continuous assay for opsonized zymosan- stimulated
granulocyte superoxide production shows that there is a lag time between
the addition of particles and the onset of detectable superoxide
production. Superoxide production is preceded by membrane potential
depolarization. Neither superoxide production nor membrane depolarization
occurs in granulocytes from patients with chronic granulomatous disease.
The extent of activation by opsonized zymosan is affected by the dose of
zymosan from 0.5 to 4.5 mg/ml, but the time necessary for activation (lag
time) is not. Similarly, the extent of depolarization but not the time
necessary for attaining maximum depolarization is concentration-dependent.
Effects of temperature, divalent cations, 2-deoxyglucose, cyanide, and
N-ethyl maleimide on superoxide production are similar for granulocytes
treated with soluble stimuli and with opsonized zymosan. Thus, zymosan
stimulates granulocytes to generate superoxide and undergo membrane
depolarization in a manner similar to that elected by soluble stimuli.
Volume 58,
Issue 5,
pp. 975-982,
11/01/1981
Copyright © 1981 by The American Society of Hematology
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