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B Lowenberg, A Hagemeijer and K Swart
Bone marrow and blood cells of a patient with acute monoblastic leukemia
with subclones marked with specific karyotypic abnormalities were
investigated. In order to more precisely evaluate the proliferative
abilities of these populations, leukemic cell enriched fractions were
prepared and incubated in two colony assays. Colony forming cells of the
disparate clones had growth advantages in different systems which shows
that their proliferation depended on the presence of selective stimulatory
factors in culture. In one assay, at diagnosis, colonies from the minor
clone were demonstrated exclusively. It is suggested that the assays
measured distinct cellular stages of myeloid differentiation and the
findings indicate that prior to diagnosis the neoplasm had evolved into
subsets with progressive dedifferentiation. Differences of growth in vitro
correlated with the different roles of these clones in the clinical history
of the disease. Approaches based on differential cloning of tumor stem
cells as in this example, may be useful for discriminating biological
properties of heterogeneous subpopulations within neoplasms, and may
facilitate the cytogenetic recognition of minimal clones among composite
malignant cell specimens.
This article has been cited by other articles:
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| Copyright © 1982 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
