Murine eosinophils labeled with indium-111 oxine: localization to delayed
hypersensitivity reactions against a schistosomal antigen and to lymphokine
in vivo
TH Rand, JA Clanton, V Runge, D English and DG Colley
We have evaluated a method for quantitation of eosinophil migration to
stimuli in vivo. Upon transfusion into normal syngeneic mice, 111In-
labeled eosinophils had an intravascular half-life of 9.5 hr and
distributed predominantly into spleen, bone marrow, and liver. In either
Schistosoma mansoni-infected mice or recipients of lymphoid cells from
infected mice, intradermal (ear pinna) injection of the schistosomal egg
antigenic preparation (SEA) elicited time-dependent accumulation of
111In-labeled eosinophils detectable by either gamma scintillation counting
of tissue samples or by nuclear medicine external imaging. Intradermal
administration of a lymphokine fraction (containing eosinophil stimulation
promoter activity) similarly caused accumulation of 111In-labeled
eosinophils. Both reactions depended on the concentration of stimulus (SEA
or lymphokine). 111In-labeled neutrophils or macrophages or 125I-albumin
did not preferentially accumulate at the reactions examined to the extent
found with 111In- labeled eosinophils, indicating that localization of
label depends on an active process and is due to eosinophils rather than a
contaminating cell type. The method was used to estimate how long
eosinotactic lymphokine remained at dermal sites: 60% of initial activity
was present 12 hr after injection. The model is discussed with regard to
the role of lymphokines in hypersensitivity reactions with eosinophil
involvement, such as the granulomatous response to S. mansoni eggs.
Volume 61,
Issue 4,
pp. 732-739,
04/01/1983
Copyright © 1983 by The American Society of Hematology
