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The response of human neutrophils to a chemotactic tripeptide (N-formyl-
methionyl-leucyl-phenylalanine) studied by microcinematography
J Maher, JV Martell, BA Brantley, EB Cox, JE Niedel and WF Rosse
A quantitative microcinematographic technique for the measurement of
cellular motion in response to chemical influences is described. The data
are analyzed by using a magnetic digitizer connected to a computer,
permitting comparison of several characteristics of such motion. When human
granulocytes are placed in a gradient of formyl-
methionyl-leucyl-phenylalanine (fMLP) (concentration 10(-6)-10(-8) mol/L)
against buffer, the motion of the cells is increased in velocity
(chemokinesis) and is directed toward the chemoattractant, as measured by
the orientation of the vector of motion. This directionality is confirmed
by positive values for the McCutcheon index and analysis of directed linear
displacement. Concentrations of fMLP below 10(-9) mol/L did not result in
chemokinesis or chemotaxis, presumably due to insufficient stimulus.
Concentrations of 10(-6) mol/L fMLP and greater resulted in marked
stimulation of the cell surface but reduced directionality and velocity of
motion compared with cells in a more optimal gradient. Motion of the
unstimulated cells tended to be ortholinear , and this was not increased by
the presence of a gradient of fMLP. Hence, as previously shown, the Random
Walk analysis, which does not give weight to the direction of motion, is
not useful in the analysis of the response of these cells to a chemotaxin
in this system.
Volume 64,
Issue 1,
pp. 221-228,
07/01/1984
Copyright © 1984 by The American Society of Hematology
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