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Changes in activities and isozyme patterns of glycolytic enzymes during
erythroid differentiation in vitro
W Nijhof, PK Wierenga, GE Staal and G Jansen
Late committed progenitor cells of erythropoiesis, CFU-E (colony- forming
unit--erythroid), were isolated from mouse spleens to near homogeneity by a
three-step enrichment procedure. The procedure included a four-day
pretreatment of bled mice with the antibiotic thiamphenicol, a recovery
period of 3 1/2 days, followed by centrifugal elutriation and Percoll
density gradient centrifugation of the spleen cells. This practically pure
CFU-E population was used to study some aspects of erythroid
differentiation in vitro. Colony growth, as well as morphology and
glycolytic enzyme activities of cells isolated at selected times of the
48-hour culture period, were determined. Marked declining activities of
several enzymes, including hexokinase, phosphofructokinase, aldolase,
enolase, pyruvate kinase, and glucose-6- phosphate dehydrogenase, were
observed during in vitro differentiation. The activity of
diphosphoglycerate mutase was almost absent in the CFU- E, but
progressively increased during differentiation. The isozyme distribution of
aldolase and enolase did not change during CFU-E in vitro differentiation
into the reticulocyte. Hexokinase (HK) in the CFU- E contained mainly a
double-banded type I isozyme, in addition to a minor amount of HK II.
During differentiation, a shift was noticed within the double-banded HK I
region, whereas HK ii disappeared after one cell division. Pyruvate kinase
in the CFU-E was characterized by the presence of both the K-type and the
L-type isozyme and hybrids of these isozyme types. During in vitro
differentiation, the production of the K-type isozyme rapidly stops in
favor of the L type.
Volume 64,
Issue 3,
pp. 607-613,
09/01/1984
Copyright © 1984 by The American Society of Hematology
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