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ID Dube, ZA Arlin, DK Kalousek, CJ Eaves and AC Eaves
We have investigated the clonality of Ph1-negative hemopoietic progenitor
cells appearing in long-term marrow cultures established with cells from a
mosaic Turner syndrome patient (46,XX/45,X) with Ph1- positive chronic
myeloid leukemia (CML). The Ph1-positive clone had been shown previously to
have arisen from a cell of the 45,X lineage. At the time of the present
study, the patient was five years post- diagnosis and had been off
chemotherapy for two months following a year of treatment for lymphoid
blast crisis. All analyzed unstimulated marrow metaphases and each of 23
individually analyzed erythroid and granulocyte colonies produced in assays
of the starting marrow were 45,X,Ph1. Pooled granulocyte colonies from the
same assays yielded four metaphases that were 45,X,Ph1 and one that was
46,XX. Very few hemopoietic progenitors were detected in long-term cultures
at any time; however, all of four individually analyzed large granulocyte
colonies and a pooled granulocyte colony preparation obtained from assays
of 4- to 6-week-old adherent layers yielded exclusively 46,XX metaphases.
These results provide evidence that non-clonal progenitors can persist in
patients with CML, even after the onset and treatment of blast crisis, and
that the long-term marrow culture system provides a sensitive method for
detecting such cells.
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| Copyright © 1984 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
