|
|
 Previous Article | Table of Contents | Next Article 
Eccentric localization of von Willebrand factor in an internal structure of
platelet alpha-granule resembling that of Weibel-Palade bodies
EM Cramer, D Meyer, R le Menn and J Breton-Gorius
Immunogold staining was used to study the ultrastructural distribution of
von Willebrand factor (vWF) in unstimulated platelets. vWF was detected in
the alpha-granules with a specific eccentric distribution pattern opposite
the nucleoids. Similar findings were obtained with a polyclonal antibody or
a pool of monoclonal antibodies to human vWF. This labeling coincided with
the presence of tubular structures located at the periphery of the
alpha-granules. These structures were better visualized on platelets
treated for standard electron microscopy: they formed a group of one to
four tubules ranging from 200 A to 250 A in diameter. They closely
resembled the internal tubular structures found in Weibel-Palade bodies,
which are the storage organelles of vWF in endothelial cells.
Volume 66,
Issue 3,
pp. 710-713,
09/01/1985
Copyright © 1985 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. N. Vomund and E. M. Majerus
ADAMTS13 Bound to Endothelial Cells Exhibits Enhanced Cleavage of von Willebrand Factor
J. Biol. Chem.,
November 6, 2009;
284(45):
30925 - 30932.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R.-H. Huang, Y. Wang, R. Roth, X. Yu, A. R. Purvis, J. E. Heuser, E. H. Egelman, and J. E. Sadler
Assembly of Weibel Palade body-like tubules from N-terminal domains of von Willebrand factor
PNAS,
January 15, 2008;
105(2):
482 - 487.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Lo, L. Li, P. Gissen, H. Christensen, P. J. McKiernan, C. Ye, M. Abdelhaleem, J. A. Hayes, M. D. Williams, D. Chitayat, et al.
Requirement of VPS33B, a member of the Sec1/Munc18 protein family, in megakaryocyte and platelet {alpha}-granule biogenesis
Blood,
December 15, 2005;
106(13):
4159 - 4166.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Kahn, T. G. Diacovo, D. F. Bainton, F. Lanza, J. Trejo, and S. R. Coughlin
Glycoprotein V-Deficient Platelets Have Undiminished Thrombin Responsiveness and Do Not Exhibit a Bernard-Soulier Phenotype
Blood,
December 15, 1999;
94(12):
4112 - 4121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Matsui, T. Shimoyama, M. Matsumoto, Y. Fujimura, Y. Takemoto, M. Sako, J. Hamako, and K. Titani
ABO Blood Group Antigens on Human Plasma von Willebrand Factor After ABO-Mismatched Bone Marrow Transplantation
Blood,
October 15, 1999;
94(8):
2895 - 2900.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P.M. Hayward, Z. Song, S. Zheng, R. Fung, M. Pai, J.-M. Masse, and E. M. Cramer
Multimerin Processing by Cells With and Without Pathways for Regulated Protein Secretion
Blood,
August 15, 1999;
94(4):
1337 - 1347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Yamamoto, V. de Waard, C. Fearns, and D. J. Loskutoff
Tissue Distribution and Regulation of Murine von Willebrand Factor Gene Expression In Vivo
Blood,
October 15, 1998;
92(8):
2791 - 2801.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. F.G. Heijnen, N. Debili, W. Vainchencker, J. Breton-Gorius, H. J. Geuze, and J. J. Sixma
Multivesicular Bodies Are an Intermediate Stage in the Formation of Platelet alpha -Granules
Blood,
April 1, 1998;
91(7):
2313 - 2325.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. M. Hayward, E. M. Cramer, Z. Song, S. Zheng, R. Fung, J.-M. Masse, R. H. Stead, and T. J. Podor
Studies of Multimerin in Human Endothelial Cells
Blood,
February 15, 1998;
91(4):
1304 - 1317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. K. Schick, J. Walker, B. Profeta, L. Denisova, and V. Bennett
Synthesis and Secretion of von Willebrand Factor and Fibronectin in Megakaryocytes at Different Phases of Maturation
Arterioscler Thromb Vasc Biol,
April 1, 1997;
17(4):
797 - 801.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. P. M. Hayward, E. Furmaniak-Kazmierczak, A.-M. Cieutat, J. C. Moore, D. F. Bainton, M. E. Nesheim, J. G. Kelton, and G. Côté
Factor V Is Complexed with Multimerin in Resting Platelet Lysates and Colocalizes with Multimerin in Platelet alpha-Granules
J. Biol. Chem.,
August 18, 1995;
270(33):
19217 - 19224.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. E. Sanders Jr, R. L. Reddick, T. C. Nichols, K. M. Brinkhous, and M. S. Read
Thrombotic Thrombocytopenia Induced in Dogs and Pigs : The Role of Plasma and Platelet vWF in Animal Models of Thrombotic Thrombocytopenic Purpura
Arterioscler Thromb Vasc Biol,
June 1, 1995;
15(6):
793 - 800.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. D. Boon
An Overview of Hemostasis
Toxicol Pathol,
January 1, 1993;
21(2):
170 - 179.
[Abstract]
[PDF]
|
|
|
|
|
