Effects of dibutyryl adenosine 3',5'-cyclic monophosphate on erythropoietin
production in human renal carcinoma cell cultures
M Hagiwara, SM Pincus, IL Chen, BS Beckman and JW Fisher
A human renal carcinoma from a patient with erythrocytosis, serially
transplanted into athymic nude mice, was grown in primary monolayer cell
cultures. After reaching confluency, the cultured cells formed
multicellular hemicysts (domes), which became more abundant as the cultures
approached saturation density. Erythropoietin (Ep) production by this renal
carcinoma in culture was only slightly increased at the time of
semiconfluency but showed a marked increase after the cultures reached
confluency, in parallel with dome formation. Dibutyryl adenosine
3',5'-cyclic monophosphate significantly (P less than .01) stimulated Ep
production and dome formation in the semiconfluent and confluent cultures
of the renal carcinoma.
Volume 66,
Issue 3,
pp. 714-717,
09/01/1985
Copyright © 1985 by The American Society of Hematology
