|
|
 Previous Article | Table of Contents | Next Article 
Inhibition of platelet adhesion to fibronectin, fibrinogen, and von
Willebrand factor substrates by a synthetic tetrapeptide derived from the
cell-binding domain of fibronectin
DM Haverstick, JF Cowan, KM Yamada and SA Santoro
The role in platelet function of the cell-binding region of fibronectin was
explored by the use of synthetic peptides. The prototypical peptide
gly-arg-gly-asp-ser was capable of inhibiting thrombin-induced platelet
aggregation without altering the degree of platelet activation as judged by
the secretion of 14C-serotonin. The peptide also effectively inhibited, in
a concentration-dependent manner, the binding of radiolabeled fibronectin
to platelets and the adhesion of platelets to fibronectin substrates. The
smallest peptide from the cell-binding region of fibronectin which retained
full activity was arg-gly-asp-ser. Transposition of amino acids or
conservative substitutions of amino acids within this short sequence
resulted in inactive peptides. Peptides containing the arg-gly-asp-ser
sequence were also capable of inhibiting the adhesion of platelets to
fibrinogen and von Willebrand factor substrates. Examination of the entire
panel of synthetic peptides for ability to inhibit adhesion to fibrinogen
or von Willebrand factor substrates revealed the same structure-function
relationships that had been determined in the studies with fibronectin.
Volume 66,
Issue 4,
pp. 946-952,
10/01/1985
Copyright © 1985 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. B. Basani, H. Zhu, M. A. Thornton, C. S. Soto, W. F. DeGrado, M. A. Kowalska, J. S. Bennett, and M. Poncz
Species differences in small molecule binding to {alpha}IIb{beta}3 are the result of sequence differences in 2 loops of the {alpha}IIb {beta} propeller
Blood,
January 22, 2009;
113(4):
902 - 910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Cierniewski, T. Byzova, M. Papierak, T. A. Haas, J. Niewiarowska, L. Zhang, M. Cieslak, and E. F. Plow
Peptide Ligands Can Bind to Distinct Sites in Integrin alpha IIbbeta 3 and Elicit Different Functional Responses
J. Biol. Chem.,
June 11, 1999;
274(24):
16923 - 16932.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-R. Sheu, M. H. Yen, W. C. Hung, Y. M. Lee, C. H. Su, and T. F. Huang
Triflavin Inhibits Platelet-Induced Vasoconstriction in De-endothelialized Aorta
Arterioscler Thromb Vasc Biol,
December 1, 1997;
17(12):
3461 - 3468.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. V. Byzova and E. F. Plow
Networking in the Hemostatic System. INTEGRIN alpha IIbbeta 3 BINDS PROTHROMBIN AND INFLUENCES ITS ACTIVATION
J. Biol. Chem.,
October 24, 1997;
272(43):
27183 - 27188.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Abraham, E. M. Nutt, R. A. Bednar, B. Bednar, R. J. Gould, and L. T. Duong
Arginine-Glycine-Aspartic Acid Mimics Can Identify a Transitional Activation State of Recombinant alpha IIbbeta 3 in Human Embryonic Kidney 293 Cells
Mol. Pharmacol.,
August 1, 1997;
52(2):
227 - 236.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. J. Kunicki, D. S. Annis, Y.-J. Deng, J. C. Loftus, and S. J. Shattil
A Molecular Basis for Affinity Modulation of Fab Ligand Binding to Integrin alpha IIbbeta 3
J. Biol. Chem.,
August 23, 1996;
271(34):
20315 - 20321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Loh, K. Beaverson, G. Vilaire, W. Qi, M. Poncz, and J. S. Bennett
Agonist-stimulated Ligand Binding by the Platelet Integrin alphaIIbbeta3 in a Lymphocyte Expression System
J. Biol. Chem.,
August 4, 1995;
270(31):
18631 - 18636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. J. Kunicki, K. R. Ely, T. C. Kunicki, Y. Tomiyama, and D. S. Annis
The Exchange of Arg-Gly-Asp (RGD) and Arg-Tyr-Asp (RYD) Binding Sequences in a Recombinant Murine Fab Fragment Specific for the Integrin [IMAGE][IMAGE][IMAGE][IMAGE] Does Not Alter Integrin Recognition
J. Biol. Chem.,
July 14, 1995;
270(28):
16660 - 16665.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Honda, Y. Tomiyama, A. J. Pelletier, D. Annis, Y. Honda, R. Orchekowski, Z. Ruggeri, and T. J. Kunicki
Topography of Ligand-induced Binding Sites, Including a Novel Cation-sensitive Epitope (AP5) at the Amino Terminus, of the Human Integrin [IMAGE][IMAGE] Subunit
J. Biol. Chem.,
May 19, 1995;
270(20):
11947 - 11954.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.H. Ginsberg, T.E. O'Toole, J.C. Loftus, and E.F. Plow
Ligand Binding to Integrins: Dynamic Regulation and Common Mechanisms
Cold Spring Harb Symp Quant Biol,
January 1, 1992;
57(0):
221 - 231.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. D'Souza, M. Ginsberg, T. Burke, S. Lam, and E. Plow
Localization of an Arg-Gly-Asp recognition site within an integrin adhesion receptor
Science,
October 7, 1988;
242(4875):
91 - 93.
[Abstract]
[PDF]
|
|
|
|
|
|
|
E Ruoslahti and M. Pierschbacher
New perspectives in cell adhesion: RGD and integrins
Science,
October 23, 1987;
238(4826):
491 - 497.
[Abstract]
[PDF]
|
|
|
|
|
|
|
R. L. Avery and B. M. Glaser
Inhibition of Retinal Pigment Epithelial Cell Attachment by a Synthetic Peptide Derived From the Cell-Binding Domain of Fibronectin
Arch Ophthalmol,
August 1, 1986;
104(8):
1220 - 1222.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J.E. Sadler, B.B. Shelton-Inloes, J.M. Sorace, and K. Titani
Cloning of cDNA and Genomic DNA for Human von Willebrand Factor
Cold Spring Harb Symp Quant Biol,
January 1, 1986;
51(0):
515 - 523.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. A. Dumin, S. K. Dickeson, T. P. Stricker, M. Bhattacharyya-Pakrasi, J. D. Roby, S. A. Santoro, and W. C. Parks
Pro-collagenase-1 (Matrix Metalloproteinase-1) Binds the alpha 2beta 1 Integrin upon Release from Keratinocytes Migrating on Type I Collagen
J. Biol. Chem.,
July 27, 2001;
276(31):
29368 - 29374.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
