|
|
 Previous Article | Table of Contents | Next Article 
Acute mixed lineage leukemia: clinicopathologic correlations and prognostic
significance
J Mirro, TF Zipf, CH Pui, G Kitchingman, D Williams, S Melvin, SB Murphy and S Stass
The frequency and clinical significance of acute leukemia displaying both
lymphoid and myeloid characteristics was determined in 123 consecutive
children using a panel of lineage-associated markers. The leukemic blasts
from 18 of 95 children (19%) with the diagnosis of acute lymphoblastic
leukemia (ALL) by standard diagnostic criteria expressed myeloid-associated
cell surface antigens. Despite immunological evidence of lymphoid
differentiation (17 CALLA + and one T cell-associated antigen +) and
findings of immunoglobulin gene rearrangement, blasts from these patients
reacted with one to five monoclonal antibodies identifying
myeloid-associated cell surface antigens (My-1, MCS.2, Mo1, SJ-D1, or 5F1).
Dual staining with microsphere-conjugated antibodies and analysis by flow
cytometry confirmed that some blasts were simultaneously expressing
lymphoid- and myeloid-associated antigens. Conversely, blasts from seven of
28 patients (25%) with acute nonlymphocytic leukemia (ANLL), diagnosed by
otherwise standard morphological and cytochemical criteria, expressed
lymphoid-associated surface antigens. Dual staining of individual blasts
demonstrated simultaneous expression of myeloperoxidase (MPO) (including
Auer rods) in association with either T-11, CALLA, or terminal
deoxynucleotidyl transferase. Blasts from one patient with ANLL
demonstrated T cell receptor gene rearrangement, while blasts from another
patient demonstrated characteristics associated with T (T-11), B (CALLA and
heavy-chain immunoglobulin gene rearrangement), and myeloid (MPO) lineage.
There were no consistent cytogenetic abnormalities, and no patient
demonstrated independent leukemic clones. Each patient with typical ALL,
except for myeloid-associated antigens, achieved complete remission with
conventional induction therapy for ALL. By contrast, three of the seven
children with ANLL whose blasts expressed the T-11 surface antigen failed
ANLL induction therapy. These three patients subsequently achieved
remission with ALL therapy.
Volume 66,
Issue 5,
pp. 1115-1123,
11/01/1985
Copyright © 1985 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. K. Slany
The molecular biology of mixed lineage leukemia
Haematologica,
July 1, 2009;
94(7):
984 - 993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Rubnitz, M. Onciu, S. Pounds, S. Shurtleff, X. Cao, S. C. Raimondi, F. G. Behm, D. Campana, B. I. Razzouk, R. C. Ribeiro, et al.
Acute mixed lineage leukemia in children: the experience of St Jude Children's Research Hospital
Blood,
May 21, 2009;
113(21):
5083 - 5089.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. M. Uckun, H. N. Sather, P. S. Gaynon, D. C. Arthur, M. E. Trigg, D. G. Tubergen, J. Nachman, P. G. Steinherz, M. G. Sensel, and G. H. Reaman
Clinical Features and Treatment Outcome of Children With Myeloid Antigen Positive Acute Lymphoblastic Leukemia: A Report From the Children's Cancer Group
Blood,
July 1, 1997;
90(1):
28 - 35.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
