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Half-lives of beta and gamma globin messenger RNAs and of protein synthetic
capacity in cultured human reticulocytes
J Ross and TD Sullivan
The turnover rates of beta and gamma globin messenger RNAs and of beta and
gamma globin protein synthesis in human reticulocytes have been measured.
Our goal was to determine whether beta globin mRNA is significantly more
stable than gamma globin mRNA during the final stages of erythroid cell
maturation. Such a result could explain the reported increase in the
beta-gamma protein synthetic ratio during erythroid maturation. As
determined by molecular hybridization and cell- free translation assays,
the half-lives of both mRNAs are 20 to 29 hours in adult and neonatal
reticulocytes. Protein synthetic capacity in intact cells decays with a
half-life of six to eight hours, but beta protein synthesis declines at the
same rate as gamma. Therefore, the changing ratio of fetal to adult
hemoglobin synthesis during late erythroid maturation does not result from
differences in mRNA turnover rates or changes in translation efficiencies.
These data, coupled with those obtained with immature erythroid cells
(Farquhar et al, Dev Biol 85: 403, 1981), suggest that, during erythroid
maturation, the gamma- beta globin protein synthesis ratio declines because
gamma gene transcription ceases earlier than beta gene transcription. Our
results also indicate that the protein synthetic machinery, not the
quantity of mRNA, becomes rate-limiting for globin production in cultured
reticulocytes.
Volume 66,
Issue 5,
pp. 1149-1154,
11/01/1985
Copyright © 1985 by The American Society of Hematology
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