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Demonstration of burst-promoting activity of recombinant human GM-CSF on
circulating erythroid progenitors using an assay involving the delayed
addition of erythropoietin
RE Donahue, SG Emerson, EA Wang, GG Wong, SC Clark and DG Nathan
We demonstrate through the use of an in vitro assay involving the delayed
addition of erythropoietin that human recombinant GM-CSF, cloned from a
mature T cell line, Mo, clearly has burst-promoting activity (BPA) on
peripheral blood erythroid progenitors at picomolar concentrations. Delay
for up to 72 hours of the addition of erythropoietin to semi-solid
methylcellulose cultures of concentrated peripheral blood progenitors
minimizes or eliminates BPA-independent erythroid colony formation with
little loss of BPA-dependent erythroid colony formation. This assay will
prove useful in accurately detecting sources of BPA.
Volume 66,
Issue 6,
pp. 1479-1481,
12/01/1985
Copyright © 1985 by The American Society of Hematology
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