The regulatory role of interleukin 2-responsive T lymphocytes on early and
mature erythroid progenitor proliferation
Z Estrov, C Roifman, YP Wang, T Grunberger, EW Gelfand and MH Freedman
To analyze the role of T lymphocytes in human erythropoiesis, we evaluated
the effect of recombinant interleukin 2 (IL 2) on marrow CFU- E and BFU-E
colony formation in vitro. IL 2 resulted in an increase in CFU-E and BFU-E
colony numbers in a dose-dependent manner. This increase could be prevented
by anti-Tac, a monoclonal antibody to the IL 2 receptor. Moreover, anti-Tac
on its own resulted in an overall decrease in colony numbers. Depletion of
marrow adherent cells did not alter the effect of either IL 2 or anti-Tac
on colony growth. Following the removal of marrow T lymphocytes, CFU-E and
BFU-E colony formation proceeded normally; however, the effects of IL 2 and
anti-Tac were markedly diminished. Readdition of T lymphocytes to the
cultures restored the IL 2 effect. Although T lymphocytes were not
themselves essential for in vitro erythropoiesis, our studies suggest that
IL 2 and IL 2-responsive T cells can regulate both early and mature stages
of erythroid differentiation.
Volume 67,
Issue 6,
pp. 1607-1610,
06/01/1986
Copyright © 1986 by The American Society of Hematology
