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E Sariban, T Mitchell and D Kufe
The murine sarcoma virus 3611 contains the transforming v-raf gene that has
partial nucleotide homology with the src family of tyrosine kinase-
encoding oncogenes. Although this virus induces fibrosarcomas in mice, a
recombinant murine retrovirus carrying both the raf and myc oncogenes
induces immunoblastic lymphomas and immortalizes mouse macrophages in
vitro. The present study has thus monitored the expression of c-raf in
human hematopoietic cells. The results demonstrate the presence of a 3.6-kb
c-raf transcript in HL-60 promyelocytic leukemic cells. The induction of
HL-60 cell differentiation along the monocytic or granulocytic lineages had
no detectable effect on the level of c-raf transcripts. Furthermore, in
contrast to c-myc and c-fms expression, inhibition of protein synthesis
with cycloheximide had no detectable effect on c-raf expression. Similar
levels of c-rafRNA were also found in other human cell lines derived from
myeloid, B cell, and T cell tumors, as well as in normal granulocytes,
monocytes, and macrophages. These findings suggest that the c-raf
protooncogene is widely expressed in multiple hematopoietic lineages.
This article has been cited by other articles:
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| Copyright © 1987 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
