|
|
 Previous Article | Table of Contents | Next Article 
Increased surface expression of the membrane glycoprotein IIb/IIIa complex
induced by platelet activation. Relationship to the binding of fibrinogen
and platelet aggregation
K Niiya, E Hodson, R Bader, V Byers-Ward, JA Koziol, EF Plow and ZM Ruggeri
Platelet activation altered the binding of three monoclonal antibodies
(monovalent Fab' fragment) directed against the glycoprotein (GP) IIb/IIIa
complex. An increased binding of two- to threefold occurred after
stimulation with thrombin or phorbol myristate acetate (PMA), with slight
but significant increase in the dissociation constants (Kd) of two
antibodies (LJ-CP8 and LJ-P9). In contrast, no statistically significant
changes were observed with ADP-stimulated platelets. The increased binding
of LJ-CP3, but not of the other two antibodies, to activated platelets
decreased by 30% to 40% in the presence of EDTA at 22 to 25 degrees C.
Platelets stimulated by thrombin or PMA bound more fibrinogen than did
those stimulated by ADP, and significant differences in the extent but not
in the affinity of fibrinogen binding were observed with various platelet
agonists. When the pool of GP IIb/IIIa molecules exposed on the surface of
unstimulated platelets was reacted with the monoclonal antibody LJ-CP3 to
block ADP-induced fibrinogen binding and platelet aggregation, stimulation
with thrombin or PMA still induced substantial binding of antibody and
fibrinogen, and aggregation ensued. Therefore, platelets exposed to
"strong" agonists exhibit an increased number of surface-oriented epitopes
associated with GP IIb/IIIa. The GP IIb/IIIa molecules bearing these newly
exposed epitopes are functional in that they can bind fibrinogen and
mediate platelet aggregation.
Volume 70,
Issue 2,
pp. 475-483,
08/01/1987
Copyright © 1987 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Mazzucato, M. R. Cozzi, M. Battiston, M. Jandrot-Perrus, M. Mongiat, P. Marchese, T. J. Kunicki, Z. M. Ruggeri, and L. De Marco
Distinct spatio-temporal Ca2+ signaling elicited by integrin {alpha}2{beta}1 and glycoprotein VI under flow
Blood,
September 24, 2009;
114(13):
2793 - 2801.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Marcondes, M. H. M. Cardoso, R. P. Morganti, S. M. Thomazzi, S. Lilla, F. Murad, G. De Nucci, and E. Antunes
Cyclic GMP-independent mechanisms contribute to the inhibition of platelet adhesion by nitric oxide donor: A role for {alpha}-actinin nitration
PNAS,
February 28, 2006;
103(9):
3434 - 3439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Chen, C.-X. Sun, and J.-N. Liu
A Novel Anti-platelet Monoclonal Antibody (3C7) Specific for the Complex of Integrin {alpha}IIb{beta}3 Inhibits Platelet Aggregation and Adhesion
J. Biol. Chem.,
July 8, 2005;
280(27):
25403 - 25408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mazzucato, M. R. Cozzi, P. Pradella, Z. M. Ruggeri, and L. De Marco
Distinct roles of ADP receptors in von Willebrand factor-mediated platelet signaling and activation under high flow
Blood,
November 15, 2004;
104(10):
3221 - 3227.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. H. Weinreb, K. J. Simon, P. Rayhorn, W. J. Yang, D. R. Leone, B. M. Dolinski, B. R. Pearse, Y. Yokota, H. Kawakatsu, A. Atakilit, et al.
Function-blocking Integrin {alpha}v{beta}6 Monoclonal Antibodies: DISTINCT LIGAND-MIMETIC AND NONLIGAND-MIMETIC CLASSES
J. Biol. Chem.,
April 23, 2004;
279(17):
17875 - 17887.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Quinn, T. V. Byzova, J. Qin, E. J. Topol, and E. F. Plow
Integrin {alpha}IIb{beta}3 and Its Antagonism
Arterioscler Thromb Vasc Biol,
June 1, 2003;
23(6):
945 - 952.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Sjobring, U. Ringdahl, and Z. M. Ruggeri
Induction of platelet thrombi by bacteria and antibodies
Blood,
December 15, 2002;
100(13):
4470 - 4477.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mazzucato, P. Pradella, M. R. Cozzi, L. De Marco, and Z. M. Ruggeri
Sequential cytoplasmic calcium signals in a 2-stage platelet activation process induced by the glycoprotein Ibalpha mechanoreceptor
Blood,
September 26, 2002;
100(8):
2793 - 2800.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Pilch, R. Habermann, and B. Felding-Habermann
Unique Ability of Integrin alpha vbeta 3 to Support Tumor Cell Arrest under Dynamic Flow Conditions
J. Biol. Chem.,
June 7, 2002;
277(24):
21930 - 21938.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. I. Litvinov, H. Shuman, J. S. Bennett, and J. W. Weisel
Binding strength and activation state of single fibrinogen-integrin pairs on living cells
PNAS,
May 28, 2002;
99(11):
7426 - 7431.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-J. Jacobin, J. Laroche-Traineau, M. Little, A. Keller, K. Peter, M. Welschof, A. Nurden, and G. Clofent-Sanchez
Human IgG Monoclonal Anti-{alpha}IIb{beta}3-Binding Fragments Derived from Immunized Donors Using Phage Display
J. Immunol.,
February 15, 2002;
168(4):
2035 - 2045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kamata, K. K. Tieu, A. Irie, T. A. Springer, and Y. Takada
Amino Acid Residues in the alpha IIb Subunit That Are Critical for Ligand Binding to Integrin alpha IIbbeta 3 Are Clustered in the beta -Propeller Model
J. Biol. Chem.,
November 16, 2001;
276(47):
44275 - 44283.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Boudreaux and D. L. Lipscomb
Clinical, Biochemical, and Molecular Aspects of Glanzmann's Thrombasthenia in Humans and Dogs
Vet. Pathol.,
May 1, 2001;
38(3):
249 - 260.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Felding-Habermann, T. E. O'Toole, J. W. Smith, E. Fransvea, Z. M. Ruggeri, M. H. Ginsberg, P. E. Hughes, N. Pampori, S. J. Shattil, A. Saven, et al.
Integrin activation controls metastasis in human breast cancer
PNAS,
February 13, 2001;
98(4):
1853 - 1858.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H. M. Smits, J. van der Linden, P. J. Blankestijn, and T. J. Rabelink
Coagulation and haemodialysis access thrombosis
Nephrol. Dial. Transplant.,
November 1, 2000;
15(11):
1755 - 1760.
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Puzon-McLaughlin, T. Kamata, and Y. Takada
Multiple Discontinuous Ligand-mimetic Antibody Binding Sites Define a Ligand Binding Pocket in Integrin alpha IIbbeta 3
J. Biol. Chem.,
March 10, 2000;
275(11):
7795 - 7802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. M. Ruggeri, J. A. Dent, and E. Saldivar
Contribution of Distinct Adhesive Interactions to Platelet Aggregation in Flowing Blood
Blood,
July 1, 1999;
94(1):
172 - 178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Miyata and Z. M. Ruggeri
Distinct Structural Attributes Regulating von Willebrand Factor A1 Domain Interaction with Platelet Glycoprotein Ibalpha under Flow
J. Biol. Chem.,
March 5, 1999;
274(10):
6586 - 6593.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Nurden, C. Poujol, C. Durrieu-Jais, J. Winckler, R. Combrie, L. Macchi, C. Bihour, C. Wagner, R. Jordan, and A. T. Nurden
Labeling of the Internal Pool of GP IIb-IIIa in Platelets by c7E3 Fab Fragments (abciximab): Flow and Endocytic Mechanisms Contribute to the Transport
Blood,
March 1, 1999;
93(5):
1622 - 1633.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Bihour, C. Durrieu-Jais, L. Macchi, C. Poujol, P. Coste, P. Besse, P. Nurden, and A. T. Nurden
Expression of Markers of Platelet Activation and the Interpatient Variation in Response to Abciximab
Arterioscler Thromb Vasc Biol,
February 1, 1999;
19(2):
212 - 219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mazzucato, P. Spessotto, A. Masotti, L. De Appollonia, M. R. Cozzi, A. Yoshioka, R. Perris, A. Colombatti, and L. De Marco
Identification of Domains Responsible for von Willebrand Factor Type VI Collagen Interaction Mediating Platelet Adhesion under High Flow
J. Biol. Chem.,
January 29, 1999;
274(5):
3033 - 3041.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. Mickelson, M. N. Ali, N. S. Kleiman, N. M. Lakkis, T. W. Chow, B. J. Hughes, and C. W. Smith
Chimeric 7e3 Fab (ReoPro) decreases detectable CD11b on neutrophils from patients undergoing coronary angioplasty
J. Am. Coll. Cardiol.,
January 1, 1999;
33(1):
97 - 106.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Peyruchaud, A.T. Nurden, S. Milet, L. Macchi, A. Pannochia, P.F. Bray, N. Kieffer, and F. Bourre
R to Q Amino Acid Substitution in the GFFKR Sequence of the Cytoplasmic Domain of the Integrin alpha IIb Subunit in a Patient With a Glanzmann's Thrombasthenia-Like Syndrome
Blood,
December 1, 1998;
92(11):
4178 - 4187.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Felding-Habermann, S. Silletti, F. Mei, C.-H. Siu, P. M. Yip, P. C. Brooks, D. A. Cheresh, T. E. O'Toole, M. H. Ginsberg, and A. M.P. Montgomery
A Single Immunoglobulin-like Domain of the Human Neural Cell Adhesion Molecule L1 Supports Adhesion by Multiple Vascular and Platelet Integrins
J. Cell Biol.,
December 15, 1997;
139(6):
1567 - 1581.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Si-Tahar, D. Pidard, V. Balloy, M. Moniatte, N. Kieffer, A. Van Dorsselaer, and M. Chignard
Human Neutrophil Elastase Proteolytically Activates the Platelet Integrin alpha IIbbeta 3 through Cleavage of the Carboxyl Terminus of the alpha IIb Subunit Heavy Chain. INVOLVEMENT IN THE POTENTIATION OF PLATELET AGGREGATION
J. Biol. Chem.,
April 25, 1997;
272(17):
11636 - 11647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Prueksaritanont, L. M. Gorham, J. A. Naue, T. G. Hamill, B. C. Askew, and K. P. Vyas
Disposition of L-738,167, A Potent and Long-Acting Fibrinogen Receptor Antagonist, in Dogs. Dose-Dependent Pharmacokinetics
Drug Metab. Dispos.,
March 1, 1997;
25(3):
355 - 361.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Yanabu, Y. Ozaki, S. Nomura, T. Miyake, Y. Miyazaki, H. Kagawa, Y. Yamanaka, N. Asazuma, K. Satoh, S. Kume, et al.
Tyrosine Phosphorylation and p72syk Activation by an Anti-Glycoprotein Ib Monoclonal Antibody
Blood,
March 1, 1997;
89(5):
1590 - 1598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kamata, A. Irie, M. Tokuhira, and Y. Takada
Critical Residues of Integrin alpha IIb Subunit for Binding of alpha IIbbeta 3 (Glycoprotein IIb-IIIa) to Fibrinogen and Ligand-mimetic Antibodies (PAC-1, OP-G2, and LJ-CP3)
J. Biol. Chem.,
August 2, 1996;
271(31):
18610 - 18615.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. C. Endenburg, L. Lindeboom-Blokzijl, J. J. Zwaginga, J. J. Sixma, and P. G. de Groot
Plasma Fibrinogen Inhibits Platelet Adhesion in Flowing Blood to Immobilized Fibrinogen
Arterioscler Thromb Vasc Biol,
May 1, 1996;
16(5):
633 - 638.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. Felding-Habermann, R. Habermann, E. Saldívar, and Z. M. Ruggeri
Role of beta3 Integrins in Melanoma Cell Adhesion to Activated Platelets under Flow
J. Biol. Chem.,
March 8, 1996;
271(10):
5892 - 5900.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Savage, E. Bottini, and Z. M. Ruggeri
Interaction of Integrin alpha[IMAGE]beta(3) with Multiple Fibrinogen Domains during Platelet Adhesion
J. Biol. Chem.,
December 1, 1995;
270(48):
28812 - 28817.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Goto, D. R. Salomon, Y. Ikeda, and Z. M. Ruggeri
Characterization of the Unique Mechanism Mediating the Shear-dependent Binding of Soluble von Willebrand Factor to Platelets
J. Biol. Chem.,
October 6, 1995;
270(40):
23352 - 23361.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. B. Addo, P. F. Bray, D. Grigoryev, N. Faraday, and P. J. Goldschmidt-Clermont
Surface Recruitment but Not Activation of Integrin {alpha}IIbß3 (GPIIb-IIIa) Requires a Functional Actin Cytoskeleton
Arterioscler Thromb Vasc Biol,
September 1, 1995;
15(9):
1466 - 1473.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. J. Pelletier, T. Kunicki, Z. M. Ruggeri, and V. Quaranta
The Activation State of the Integrin [IMAGE][IMAGE][IMAGE][IMAGE] Affects Outside-in Signals Leading to Cell Spreading and Focal Adhesion Kinase Phosphorylation
J. Biol. Chem.,
July 28, 1995;
270(30):
18133 - 18140.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Kodandapani, B. Veerapandian, T. J. Kunicki, and K. R. Ely
Crystal Structure of the OPG2 Fab
J. Biol. Chem.,
February 3, 1995;
270(5):
2268 - 2273.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. S. Nicholson, S. G. Panzer-Knodle, A. K. Salyers, B. B. Taite, J. A. Szalony, N. F. Haas, L. W. King, J. A. Zablocki, B. T. Keller, K. Broschat, et al.
SC-54684A: An Orally Active Inhibitor of Platelet Aggregation
Circulation,
January 15, 1995;
91(2):
403 - 410.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. L. Yap, S. C. Hughan, S. L. Cranmer, W. S. Nesbitt, M. M. Rooney, S. Giuliano, S. Kulkarni, S. M. Dopheide, Y. Yuan, H. H. Salem, et al.
Synergistic Adhesive Interactions and Signaling Mechanisms Operating between Platelet Glycoprotein Ib/IX and Integrin alpha IIbbeta 3. STUDIES IN HUMAN PLATELETS AND TRANSFECTED CHINESE HAMSTER OVARY CELLS
J. Biol. Chem.,
December 22, 2000;
275(52):
41377 - 41388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Soslau, R. Class, D. A. Morgan, C. Foster, S. T. Lord, P. Marchese, and Z. M. Ruggeri
Unique Pathway of Thrombin-induced Platelet Aggregation Mediated by Glycoprotein Ib
J. Biol. Chem.,
June 8, 2001;
276(24):
21173 - 21183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
