|
|
 Previous Article | Table of Contents | Next Article 
Conformational states of vitronectin: preferential expression of an
antigenic epitope when vitronectin is covalently and noncovalently
complexed with thrombin-antithrombin III or treated with urea
BR Tomasini and DF Mosher
Department of Medicine, University of Wisconsin, Madison 53706.
A difference in recognition of the adhesive glycoprotein vitronectin (also
called S-protein, serum spreading factor, and epibolin) by monoclonal
antibody 8E6 (Hayman EG, et al, Proc Natl Acad Sci USA 80:4003, 1983) was
investigated using a competitive enzyme- immunosorbent assay and
immunoaffinity chromatography. Recognition of vitronectin in serum was
approximately 50-fold greater than recognition of vitronectin in plasma.
Recognition of vitronectin incubated with heparin, thrombin-antithrombin
III complex, or heparin and thrombin- antithrombin III complex together was
2.5-, 7-, or 32-fold greater, respectively, than recognition of vitronectin
alone. Thrombin or antithrombin III by itself did not induce the antigenic
change. Factor Xa-antithrombin III was less effective than
thrombin-antithrombin III in induction of the change. Dextran sulfate and
fucoidan were more potent than heparin in induction of the antigenic
change, whereas dermatan sulfate, hyaluronic acid, heparan sulfate,
chondroitin sulfate, or keratan sulfate were less effective. Immunoblotting
analysis of serum and of vitronectin incubated with thrombin and
antithrombin III demonstrated the presence of complexes composed of
vitronectin and thrombin-antithrombin III that could only be dissociated
with reducing agent. N-ethylmaleimide completely blocked the formation of
the presumably disulfide-bonded complexes and partially blocked the
antigenic change. Both non-disulfide-bonded and disulfide-bonded
vitronectin bound to antibody-Sepharose from a mixture of vitronectin and
thrombin-antithrombin III. Treatment of vitronectin with 8 mol/L urea
resulted in enhanced recognition by the monoclonal antibody. Thus, the 8E6
antibody reacts with an epitope that is preferentially expressed by
noncovalently and covalently linked vitronectin/thrombin-antithrombin III
complexes and by urea-treated vitronectin. The change in vitronectin
induced by thrombin-antithrombin III, therefore, is a physiological
correlate of urea treatment and of adsorption of vitronectin onto tissue
culture plastic (as is done in cell adhesion assays). The change may be
important for expression of vitronectin activity.
Volume 72,
Issue 3,
pp. 903-912,
09/01/1988
Copyright © 1988 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Sangaletti, E. Di Carlo, S. Gariboldi, S. Miotti, B. Cappetti, M. Parenza, C. Rumio, R. A. Brekken, C. Chiodoni, and M. P. Colombo
Macrophage-Derived SPARC Bridges Tumor Cell-Extracellular Matrix Interactions toward Metastasis
Cancer Res.,
November 1, 2008;
68(21):
9050 - 9059.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. H. Minor, C. R. Schar, G. E. Blouse, J. D. Shore, D. A. Lawrence, P. Schuck, and C. B. Peterson
A Mechanism for Assembly of Complexes of Vitronectin and Plasminogen Activator Inhibitor-1 from Sedimentation Velocity Analysis
J. Biol. Chem.,
August 5, 2005;
280(31):
28711 - 28720.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Okumura, Y. Kamikubo, S. A. Curriden, J. Wang, T. Kiwada, S. Futaki, K. Kitagawa, and D. J. Loskutoff
Kinetic Analysis of the Interaction between Vitronectin and the Urokinase Receptor
J. Biol. Chem.,
March 8, 2002;
277(11):
9395 - 9404.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. J. Podor, S. Campbell, P. Chindemi, D. M. Foulon, D. H. Farrell, P. D. Walton, J. I. Weitz, and C. B. Peterson
Incorporation of Vitronectin into Fibrin Clots. EVIDENCE FOR A BINDING INTERACTION BETWEEN VITRONECTIN AND gamma A/gamma ' FIBRINOGEN
J. Biol. Chem.,
February 22, 2002;
277(9):
7520 - 7528.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Stupack and D. A. Cheresh
Get a ligand, get a life: integrins, signaling and cell survival
J. Cell Sci.,
January 10, 2002;
115(19):
3729 - 3738.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. P. Francois, K. T. Preissner, M. Herrmann, R. P. Haugland, P. Vaudaux, D. P. Lew, and K.-H. Krause
Vitronectin Interaction with Glycosaminoglycans. KINETICS, STRUCTURAL DETERMINANTS, AND ROLE IN BINDING TO ENDOTHELIAL CELLS
J. Biol. Chem.,
December 31, 1999;
274(53):
37611 - 37619.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. P. Fay, A. C. Parker, M. N. Ansari, X. Zheng, and D. Ginsburg
Vitronectin Inhibits the Thrombotic Response to Arterial Injury in Mice
Blood,
March 15, 1999;
93(6):
1825 - 1830.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. D. Gibson, J. A. Lamerdin, P. Zhuang, K. Baburaj, E. H. Serpersu, and C. B. Peterson
Orientation of Heparin-binding Sites in Native Vitronectin. ANALYSES OF LIGAND BINDING TO THE PRIMARY GLYCOSAMINOGLYCAN-BINDING SITE INDICATE THAT PUTATIVE SECONDARY SITES ARE NOT FUNCTIONAL
J. Biol. Chem.,
March 5, 1999;
274(10):
6432 - 6442.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Wells and M. A. Blajchman
In Vivo Clearance of Ternary Complexes of Vitronectin-Thrombin-Antithrombin Is Mediated by Hepatic Heparan Sulfate Proteoglycans
J. Biol. Chem.,
September 4, 1998;
273(36):
23440 - 23447.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Memmo and P McKeown-Longo
The alphavbeta5 integrin functions as an endocytic receptor for vitronectin
J. Cell Sci.,
January 2, 1998;
111(4):
425 - 433.
[Abstract]
[PDF]
|
|
|
|
|
|
|
D. Seiffert and J. W. Smith
The Cell Adhesion Domain in Plasma Vitronectin Is Cryptic
J. Biol. Chem.,
May 23, 1997;
272(21):
13705 - 13710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Seiffert and D. Seiffert
The Glycosaminoglycan Binding Site Governs Ligand Binding to the Somatomedin B Domain of Vitronectin
J. Biol. Chem.,
April 11, 1997;
272(15):
9971 - 9978.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Zhuang, A. I. Chen, and C. B. Peterson
Native and Multimeric Vitronectin Exhibit Similar Affinity for Heparin. DIFFERENCES IN HEPARIN BINDING PROPERTIES INDUCED UPON DENATURATION ARE DUE TO SELF-ASSOCIATION INTO A MULTIVALENT FORM
J. Biol. Chem.,
March 14, 1997;
272(11):
6858 - 6867.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Seiffert and D. J. Loskutoff
Type 1Plasminogen Activator Inhibitor Induces Multimerization of Plasma Vitronectin. A SUGGESTED MECHANISM FOR THE GENERATION OF THE TISSUE FORM OF VITRONECTIN IN VIVO
J. Biol. Chem.,
November 22, 1996;
271(47):
29644 - 29651.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Zhuang, M. N. Blackburn, and C. B. Peterson
Characterization of the Denaturation and Renaturation of Human Plasma Vitronectin. I. BIOPHYSICAL CHARACTERIZATION OF PROTEIN UNFOLDING AND MULTIMERIZATION
J. Biol. Chem.,
June 14, 1996;
271(24):
14323 - 14332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Zhuang, H. Li, J. G. Williams, N. V. Wagner, D. Seiffert, and C. B. Peterson
Characterization of the Denaturation and Renaturation of Human Plasma Vitronectin. II. INVESTIGATION INTO THE MECHANISM OF FORMATION OF MULTIMERS
J. Biol. Chem.,
June 14, 1996;
271(24):
14333 - 14343.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. S. Panetti, S. A. Wilcox, C. Horzempa, and P. J. McKeown-Longo
alpha(v)beta(5)Integrin Receptor-mediated Endocytosis of Vitronectin Is Protein Kinase C-dependent
J. Biol. Chem.,
August 4, 1995;
270(31):
18593 - 18597.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ribeiro, S Schultz-Cherry, and J. Murphy-Ullrich
Heparin-binding vitronectin up-regulates latent TGF-beta production by bovine aortic endothelial cells
J. Cell Sci.,
January 4, 1995;
108(4):
1553 - 1561.
[Abstract]
[PDF]
|
|
|
|
|
|
|
P. Underwood, J. Steele, and B. Dalton
Effects of polystyrene surface chemistry on the biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies
J. Cell Sci.,
January 3, 1993;
104(3):
793 - 803.
[Abstract]
[PDF]
|
|
|
|
|
|
|
T. J. Podor, S. G. Shaughnessy, M. N. Blackburn, and C. B. Peterson
New Insights into the Size and Stoichiometry of the Plasminogen Activator Inhibitor Type-1{middle dot}Vitronectin Complex
J. Biol. Chem.,
August 11, 2000;
275(33):
25402 - 25410.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
