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Detection of occult follicular lymphoma by specific DNA amplification
M Stetlet-Stevenson, M Raffeld, P Cohen and J Cossman
Laboratory of Pathology, National Cancer Institute, Bethesda, MD 20892.
To detect occult lymphoma, the polymerase chain reaction (PCR) technique
was used to amplify joined bcl-2/JH DNA sequences at the juncture of the
t(14:18) translocation in follicular lymphoma. Using the heat-stable DNA
polymerase Taq and automated cycling of the reaction, we were able to
detect as few as one to two copies of bcl- 2/JH. Under these conditions,
PCR proved to be at least 10,000-fold more sensitive than either
conventional flow cytometry or Southern blot restriction analysis. In
addition, genomic DNA sequences of four lymphomas confirmed that the size
of the amplified segment serves as a tumor marker. Direct application of
PCR to patient staging revealed occult malignant lymphoma in tissue
otherwise considered uninvolved by standard criteria. We conclude that the
striking enhancement in diagnostic sensitivity attained by DNA
amplification can serve as a valuable adjunct to the staging and clinical
monitoring of follicular lymphoma.
Volume 72,
Issue 5,
pp. 1822-1825,
11/01/1988
Copyright © 1988 by The American Society of Hematology
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