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Two phases of engraftment established by serial bone marrow transplantation
in mice
RJ Jones, P Celano, SJ Sharkis and LL Sensenbrenner
Johns Hopkins Oncology Center, Baltimore, MD 21205.
Serially transplanted bone marrow eventually fails to reconstitute lethally
irradiated mice. The reasons for this loss of repopulating ability are
unknown. We showed that serial bone marrow transplantation changed the
ratio of hematopoietic progenitors in bone marrow. The numbers of
granulocyte-macrophage colony-forming units (CFU-GM) in the bone marrow did
not change with serial transplantation. Spleen CFU (CFU- S) numbers
decreased with serial transfer but remained at levels which should be
associated with engraftment, even on the transfers which were unsuccessful.
The CFU-S, therefore, did not appear to be the cells responsible for
long-term hematopoietic repopulation. The number of successful serial
transfers was dependent on the size of the grafts, and prolonging the time
interval between transfers reestablished the ability of the serially
transplanted marrow to reconstitute lethally irradiated recipients. Serial
bone marrow transplantation dissociated two phases of engraftment. The
first unsustained phase was maintained with repeated serial transfer and
appeared to be produced by committed progenitors. The second sustained
phase was eventually lost with repeated serial transfer and was apparently
due to the pluripotent stem cell.
Volume 73,
Issue 2,
pp. 397-401,
02/01/1989
Copyright © 1989 by The American Society of Hematology
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