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Differential expression of MHC class II antigens in myelomonocytic leukemia
cell lines
JJ Yunis, H Band, F Bonneville and EJ Yunis
Division of Immunogenetics, Dana-Farber Cancer Institute, Boston, MA 02115.
Major histocompatibility complex (MHC) class II antigens are discordantly
expressed on hematopoietic progenitor cells. Their expression is linked to
differential responsiveness of the cells to growth factors and inhibitors.
We examined the expression of different MHC class II antigens in a panel of
human myelomonocytic cell lines representing different stages of
differentiation, by cytofluorographic analysis with monoclonal antibody
(MoAb) and Northern blot analysis with specific cDNA probes. These analyses
revealed discordant expression of different MHC class II antigens both in
basal state and after gamma-IFN induction. Thus KG-1 myeloblast cells
express all class II antigens (DR greater than DP greater than DQ)
constitutively and their expression increased after gamma-IFN treatment.
KG-1a, an immature blast variant of KG-1, does not express class II
antigens, even after gamma-IFN treatment. THP-1, a monocytic cell line
expresses DR but not DP or DQ under basal conditions. DP and DQ are,
however, gamma-IFN inducible. The class II negative HL-60 promyelocytic
cell line, expresses DR and DP but not DQ after gamma-IFN induction. In all
the above cell lines, surface expression of class II antigens correlated
with the levels of mRNA expression as determined with specific cDNA probes.
In U-937, a monocytic cell line, no surface expression of class II MHC
antigens was observed either with or without gamma-IFN, however, specific
mRNA message was observed under basal conditions and was further increased
with gamma-IFN, indicating a possible defect in assembly or transport of
class II antigens. The patterns of class II MHC antigens in these leukemic
cell lines may be a useful model to delineate molecular basis of discordant
MHC class II expression during myelomonocytic differentiation.
Volume 73,
Issue 4,
pp. 931-937,
03/01/1989
Copyright © 1989 by The American Society of Hematology
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