Expression of human interleukin-3 (multi-CSF) is restricted to human
lymphocytes and T-cell tumor lines
CM Niemeyer, CA Sieff, B Mathey-Prevot, JZ Wimperis, BE Bierer, SC Clark and DG Nathan
Division of Hematology and Pediatric Oncology, Children's Hospital, Boston,
Massachusetts.
While the cellular sources for granulocyte-macrophage colony- stimulating
factor (GM-CSF) are known to be widely distributed among several cell
types, interleukin-3 (IL-3) gene expression has been demonstrated in only
certain T-cell clones and in blood mononuclear cells stimulated with
phytohemagglutinin (PHA) and phorbol-myristate- acetate (PMA). To determine
which blood cells were responsible for this expression, we fractionated
PHA/PMA-stimulated mononuclear cells and identified T lymphocytes as the
source of IL-3 mRNA. Low-level IL-3 expression was detected as well in
several stimulated human T-cell lines. Hematopoietic stromal cells such as
fibroblasts and endothelial cells could not be induced to express IL-3
mRNA. The kinetics of IL-3 mRNA induction in mononuclear cells and
lymphocytes stimulated with PHA/PMA or anti-CD3 monoclonal antibody (MoAb)
and interleukin-1 (IL-1) were similar to those observed for GM-CSF
expression.
Volume 73,
Issue 4,
pp. 945-951,
03/01/1989
Copyright © 1989 by The American Society of Hematology
