Establishment and characterization of a childhood T-cell acute
lymphoblastic leukemia cell line, PER-255, with chromosome abnormalities
involving 7q32-34 in association with T-cell receptor- beta gene
rearrangement
UR Kees, R Lukeis, J Ford and OM Garson
Leukaemia Research Laboratory, Princess Margaret Hospital, Perth, Western
Australia.
A human leukemia cell line, PER-255, was established from the bone marrow
of a 5-year-old boy with features typical of lymphomatous T- acute
lymphoblastic leukemia (T-ALL). The leukemic origin of cell line PER-255 is
indicated by its cytochemical and immunologic similarity to the patient's
fresh leukemic cells, which correspond to immature cortical thymocytes.
Southern blot analysis showed that the IgJH genes were in germline
configuration, whereas both alleles of the T-cell receptor-beta (TCR-beta)
gene were rearranged in PER-255 cells, with identical rearrangements
present in the patient's leukemic cells. Cytogenetic analysis of the cell
line revealed a single abnormal clone with the karyotype
46,XY,t(7;10)(q32-34;q24),t(9;12) (p22;p12-13). Reciprocal translocations
involving chromosome bands 7q32-36, containing the gene for the TCR-beta
chain, have been reported for a number of tumors of T-cell origin.
Translocations involving the 7q32-36 region appear to be nonrandomly
associated with childhood T-ALL, whereas abnormalities of 9p and 12p have
been reported to be nonrandomly involved in ALL but not specifically
associated with the T- cell phenotype.
Volume 74,
Issue 1,
pp. 369-373,
07/01/1989
Copyright © 1989 by The American Society of Hematology
