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Expression of c-jun protooncogene in human myelomonocytic cells
A Bertani, N Polentarutti, A Sica, A Rambaldi, A Mantovani and F Colotta
Instituto di Ricerche Farmacologiche Mario Negri, Milano Italy.
A prototypic "immediate early" gene, c-fos, has been extensively
investigated in relation to the differentiation and activation of
myelomonocytic cells. The c-fos gene product is associated in
transcriptional complexes with the c-jun product. These protooncogenes are
part of the regulatory network of gene expression. The present study was
designed to investigate expression of the c-jun protooncogene in human
circulating myelomonocytic cells. We found that c-jun is constitutively
expressed in normal monocytes and granulocytes, whereas low levels of
transcripts are found in lymphocytes. Acute myelogenous leukemia (AML)
samples of French-American-British Cooperative Group (FAB) subtypes 1
through 4 express appreciable levels of this protooncogene. Normal
phytohemagglutinin (PHA)-activated lymphocytes express high levels of
c-jun. Expression in normal myelomonocytic cells is detectable even after
18 hours of culture. The c-jun transcripts in myelomonocytic cells have a
half-life of approximately 20 minutes and are superinduced by
cycloheximide, which affects both the degradation rate of mRNA and the
transcriptional activity of the c-jun gene. Functional activation of
monocytes and granulocytes with phorbol esters, lipopolysaccharide, and
tumor necrosis factor (TNF) increase c- jun expression. This induction is
rapid, transient, and does not require intervening protein synthesis.
Runoff experiments showed that in freshly isolated untreated monocytes, the
c-jun gene is constitutively transcribed, and that induction by
lipopolysaccharide is at least in part at the transcriptional level.
Moreover, lipopolysaccharide (LPS) treatment reduced the degradation rate
of c- jun transcripts, prolonging the half-life to approximately two hours.
Expression of c-jun in resting and activated monocytes and granulocytes
suggests that this protooncogene may play a role in the differentiation and
activation of cells belonging to the myelomonocytic lineage.
Volume 74,
Issue 5,
pp. 1811-1816,
10/01/1989
Copyright © 1989 by The American Society of Hematology
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