Spleen stromal cell lines selectively support erythroid colony formation
N Yanai, Y Matsuya and M Obinata
Department of Cell Biology, Tohoku University, Sendai, Japan.
Mouse stromal cell lines (MSS lines) have been established from the spleens
of newborn mice in culture at a low serum concentration. These MSS lines
support the proliferation and differentiation of the erythroid progenitor
cells from mouse fetal livers and bone marrow in a semisolid medium in the
presence of erythropoietin. Larger colonies of over 1,000
benzidine-positive erythroid cells were developed from the fetal liver
cells on the MSS cell layers after 6 days of incubation. These layers also
support the maturation of the erythroid cells since the enucleation process
of the latter was observed in large erythroid colonies. Metabolically
active MSS cells are apparently required to support the proliferation and
differentiation of the erythroid progenitor cells, because neither the MSS
cells inactivated with fixation nor the conditioned media of MSS cells
promoted the erythroid colony formation. These studies demonstrate that MSS
lines specifically support the proliferation and differentiation of the
erythroid progenitor cells in vitro and that stroma cells may have a
critical function in blood formation in the mouse spleen.
Volume 74,
Issue 7,
pp. 2391-2397,
11/15/1989
Copyright © 1989 by The American Society of Hematology
