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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Anderson, D. W. Articles by Pierik, L. T. Search for Related Content PubMed PubMed Citation Articles by Anderson, D. W. Articles by Pierik, L. T. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Serological confirmation of human T-lymphotropic virus type I infection in healthy blood and plasma donors DW Anderson, JS Epstein, TH Lee, MD Lairmore, C Saxinger, VS Kalyanaraman, D Slamon, W Parks, BJ Poiesz and LT Pierik Center for Biologics Evaluation and Review, FDA, Bethesda, MD 20892. We wished to develop criteria for serological confirmation of human T- lymphotropic virus type I (HTLV-I) infection in healthy donors. Selected serum or plasma samples reactive by HTLV-I enzyme immunosorbent assay or gel-agglutination assays with at least one viral- specific band on Western immunoblot (WIB) were tested in six laboratories by four WIBs and four radioimmunoprecipitation assays (RIPAs) for antibodies to HTLV-I proteins encoded by gag (p19 and p24), env (gp46 and/or gp61), and tax (p40x) genes. One hundred forty-two donor sera were obtained from 38 Japanese, 69 American, and 35 Caribbean blood or plasma donors. Among these samples, WIB assays appeared more sensitive to p24 antibodies, whereas RIPAs were significantly more sensitive to gp61 antibodies. All sera (137) with gp61 antibodies had p24 antibodies. Of the 137 sera positive for p24 and gp61 antibodies, p19 antibodies were detected in 129 sera, and p40x antibodies were detected in 108. In sera with p19 antibodies and antibodies to env- or tax-encoded proteins, p24 antibodies were always present. Antibodies to p40x were not found in the absence of gp61 antibodies. Virological evidence of infection was found in seven American donors by lymphocyte coculture (one HTLV-I, one HTLV-II) or by polymerase chain reaction (three HTLV-I, two HTLV-II). Sera from all seven donors showed p24 and gp46 and/or gp61 antibodies. We suggest that seroreactivity to both p24 and gp46 and/or gp61 by WIB or RIPA or both are suitable criteria to confirm but not to distinguish HTLV-I and HTLV-II infections. Volume 74, Issue 7, pp. 2585-2591, 11/15/1989 Copyright © 1989 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. E. Nelson, J. G. Donahue, A. Munoz, N. D. Cohen, P. M. Ness, A. Teague, V. A. Stambolis, D. H. Yawn, B. Callicott, H. McAllister, et al. Transmission of Retroviruses from Seronegative Donors by Transfusion during Cardiac Surgery: A Multicenter Study of HIV-1 and HTLV-I/II Infections Ann Intern Med, October 1, 1992; 117(7): 554 - 559. [Abstract] [PDF]

	Copyright © 1989 by American Society of Hematology         Online ISSN: 1528-0020