Receptor expression and functional status of cultured human eosinophils
derived from umbilical cord blood mononuclear cells
GM Walsh, A Hartnell, R Moqbel, O Cromwell, L Nagy, B Bradley, T Furitsu, T Ishizaka and AB Kay
Department of Allergy and Clinical Immunology, National Heart and Lung
Institute, London, UK.
Selective use of recombinant human cytokines has enabled the culture of
large numbers of eosinophils from human cord blood mononuclear cells,
raising the possibility of their use as a model of eosinophil function.
Cultured eosinophils (CE) were compared with normal-density peripheral
blood eosinophils (PBE) in terms of their membrane receptor expression and
function. Fc gamma R and CR1 expression of CE and PBE was similar. In
contrast, the specific mean fluorescence for LFA-1 alpha, p150,95 alpha,
ICAM-1, and HLA-DR was significantly elevated for CE compared with PBE. CE
responded in PAF-induced chemotaxis in a similar fashion to PBE. CE gave
higher numbers of both resting and platelet activating factor
(PAF)-stimulated immunoglobulin G (IgG)- and C3b-dependent rosettes than
PBE. CE and PBE had comparable capacity to kill IgG- and C-opsonized
schistosomula in terms of both baseline values and PAF- induced enhancement
of cytotoxicity. Baseline adherence by CE and PBE to plasma-coated glass
was essentially the same, but stimulated adhesion (PAF) of CE was lower.
Compared with PBE, CE generated less than half the amounts of extracellular
and cell-associated PAF induced by calcium ionophore A23187 stimulation.
Unlike PBE, CE did not generate PAF after exposure to IgG-coated Sepharose
particles. CE stimulated with IgG-coated beads generated small quantities
of LTC4, while A23187 stimulation resulted in approximately half the LTC4
levels observed with PBE. The total cell content of eosinophil peroxidase
(EPO) was similar for CE and PBE. These data suggest that although CE and
PBE have many phenotypic and functional properties in common there are
quantitative differences that may be a consequence of their immaturity
and/or the influence of the cytokines used in their culture.
Volume 76,
Issue 1,
pp. 105-111,
07/01/1990
Copyright © 1990 by The American Society of Hematology
