|
|
 Previous Article | Table of Contents | Next Article 
Characteristics of quinine- and quinidine-induced antibodies specific for
platelet glycoproteins IIb and IIIa
GP Visentin, PJ Newman and RH Aster
Blood Center of Southeastern Wisconsin, Milwaukee 53233.
Recent studies have shown that antibodies characteristic of quinine- and
quinidine-induced thrombocytopenia sometimes recognize the platelet
membrane glycoprotein (GP) complex IIb/IIIa in addition to their well known
target, GPIb/IX. We have investigated the frequency with which drug-induced
antibodies bind to GPIIb/IIIa and the nature of their target epitopes. In
studies of sera from 13 patients sensitive to quinidine or quinine, we
found that 10 contained IgG antibodies specific for both GPIb/IX and
GPIIb/IIIa, two reacted with GPIb/IX alone, and one reacted with GPIIb/IIIa
alone. In all cases, the presence of drug was required for binding of IgG
to target GPs. By immunoabsorption, we found that each of five polyspecific
sera contained at least two different antibodies, one reactive with GPb/IX
and the other with GPIIb/IIIa. Further studies with eight drug- dependent
antibodies (DDAb) specific for GPIIb/IIIa showed that three recognized the
GPIIb/IIIa complex only, one recognized GPIIb alone, and three recognized
GPIIIa alone. The eighth serum appeared to bind to both GPIIIa alone and to
an epitope determined by the GPIIb/IIIa complex. The three antibodies
specific for GPIIIa alone also reacted with GPIIIa deglycosylated with
endo-H, and with the major (61 Kd) fragment obtained by chymotryptic
digestion of GPIIIa but failed to react with reduced GPIIIa. These findings
demonstrate that, in drug- induced, immunologic thrombocytopenia, the
anti-platelet immune response is typically directed against epitopes on
both GPIb/IX and GPIIb/IIIa. The three DDAb we studied that were specific
for GPIIIa alone recognize epitopes resistant to chymotrypsin and endo-H
treatment that are dependent on intrachain disulfide bonding.
Volume 77,
Issue 12,
pp. 2668-2676,
06/15/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. W. Bougie, J. Birenbaum, M. Rasmussen, M. Poncz, and R. H. Aster
Quinine-dependent, platelet-reactive monoclonals mimic antibodies found in patients with quinine-induced immune thrombocytopenia
Blood,
January 29, 2009;
113(5):
1105 - 1111.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Peterson, T. N. Nelson, A. J. Kanack, and R. H. Aster
Fine specificity of drug-dependent antibodies reactive with a restricted domain of platelet GPIIIA
Blood,
February 1, 2008;
111(3):
1234 - 1239.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. H. Aster and D. W. Bougie
Drug-Induced Immune Thrombocytopenia
N. Engl. J. Med.,
August 9, 2007;
357(6):
580 - 587.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Von Drygalski, B. R. Curtis, D. W. Bougie, J. G. McFarland, S. Ahl, I. Limbu, K. R. Baker, and R. H. Aster
Vancomycin-Induced Immune Thrombocytopenia
N. Engl. J. Med.,
March 1, 2007;
356(9):
904 - 910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. W. Bougie, P. R. Wilker, and R. H. Aster
Patients with quinine-induced immune thrombocytopenia have both "drug-dependent" and "drug-specific" antibodies
Blood,
August 1, 2006;
108(3):
922 - 927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Reddy, M. A. Shuman, and R. H. Aster
Quinine/Quinidine-Induced Thrombocytopenia: A Great Imitator
Arch Intern Med,
January 26, 2004;
164(2):
218 - 220.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Asvadi, Z. Ahmadi, and B. H. Chong
Drug-induced thrombocytopenia: localization of the binding site of GPIX-specific quinine-dependent antibodies
Blood,
September 1, 2003;
102(5):
1670 - 1677.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Peterson, C. E. Nyree, P. J. Newman, and R. H. Aster
A site involving the "hybrid" and PSI homology domains of GPIIIa (beta 3-integrin subunit) is a common target for antibodies associated with quinine-induced immune thrombocytopenia
Blood,
February 1, 2003;
101(3):
937 - 942.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. R. Curtis, J. Swyers, A. Divgi, J. G. McFarland, and R. H. Aster
Thrombocytopenia after second exposure to abciximab is caused by antibodies that recognize abciximab-coated platelets
Blood,
March 15, 2002;
99(6):
2054 - 2059.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Rabetoy, M. Hansen, G. Brosnahan, and L. Hartung
The lady who had muscle cramps and developed thrombotic microangiopathy
Nephrol. Dial. Transplant.,
September 1, 2000;
15(9):
1464 - 1467.
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kroll, Q.-H. Sun, and S. Santoso
Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a target glycoprotein in drug-induced thrombocytopenia
Blood,
August 15, 2000;
96(4):
1409 - 1414.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Bednar, J. J. Cook, M. A. Holahan, M. E. Cunningham, P. A. Jumes, R. A. Bednar, G. D. Hartman, and R. J. Gould
Fibrinogen Receptor Antagonist-Induced Thrombocytopenia in Chimpanzee and Rhesus Monkey Associated With Preexisting Drug-Dependent Antibodies to Platelet Glycoprotein IIb/IIIa
Blood,
July 15, 1999;
94(2):
587 - 599.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Gentilini, B.R. Curtis, and R.H. Aster
An Antibody From a Patient With Ranitidine-Induced Thrombocytopenia Recognizes a Site on Glycoprotein IX That Is a Favored Target for Drug-Induced Antibodies
Blood,
October 1, 1998;
92(7):
2359 - 2365.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. Burgess, J. A. Lopez, M. C. Berndt, I. Dawes, C. N. Chesterman, and B. H. Chong
Quinine-Dependent Antibodies Bind a Restricted Set of Epitopes on the Glycoprotein Ib-IX Complex: Characterization of the Epitopes
Blood,
October 1, 1998;
92(7):
2366 - 2373.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Peterson, G. P. Visentin, P. J. Newman, and R. H. Aster
A Recombinant Soluble Form of the Integrin alpha IIbbeta 3 (GPIIb-IIIa) Assumes an Active, Ligand-Binding Conformation and Is Recognized by GPIIb-IIIa-Specific Monoclonal, Allo-, Auto-, and Drug-Dependent Platelet Antibodies
Blood,
September 15, 1998;
92(6):
2053 - 2063.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Hou, E. Horney, D. Stockelberg, S. Jacobsson, J. Kutti, and H. Wadenvik
Multiple Quinine-Dependent Antibodies in a Patient With Episodic Thrombocytopenia, Neutropenia, Lymphocytopenia, and Granulomatous Hepatitis
Blood,
December 15, 1997;
90(12):
4806 - 4811.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D.B. Cines and M. Laposata
Case 15-1995- A 70-year-old woman with atrial fibrillation and the rapid onset of hemorrhagic manifestations
N. Engl. J. Med.,
May 18, 1995;
332(20):
1363 - 1370.
[Full Text]
[PDF]
|
|
|
|
|
