Enhancement of the grafting efficiency of transplanted marrow cells by
preincubation with interleukin-3 and granulocyte-macrophage colony-
stimulating factor
M Tavassoli, M Konno, Y Shiota, E Omoto, JJ Minguell and ED Zanjani
University of Mississippi School of Medicine.
To improve the grafting efficiency of transplanted murine hematopoietic
progenitors, we briefly preincubated mouse bone marrow cells with
interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor
(GM-CSF) ex vivo before their transplantation into irradiated recipients.
This treatment was translated into an increase in the seeding efficiency of
colony-forming unit-spleen (CFU-S) and CFU-GM after transplantation. Not
only was the concentration of CFU-S in the tibia increased 2 and 24 hours
after transplantation, but the total cell number and CFU-S and CFU-GM
concentrations were persistently higher in IL-3- and GM-CSF-treated groups
1 to 3 weeks after transplantation. In addition, the survival of animals as
a function of transplanted cell number was persistently higher in IL-3- and
GM-CSF- treated groups compared with controls. The data indicate that the
pretreatment of marrow cells with IL-3 and GM-CSF before transplantation
increases the seeding efficiency of hematopoietic stem cells and probably
other progenitor cells after transplantation. This increased efficiency may
be mediated by upward modulation of homing receptors. Therefore, ex vivo
preincubation of donor marrow cells with IL-3 and GM-CSF may be a useful
tactic in bone marrow transplantation.
Volume 77,
Issue 7,
pp. 1599-1606,
04/01/1991
Copyright © 1991 by The American Society of Hematology
