|
|
 Previous Article | Table of Contents | Next Article 
Cloning of the human erythropoietin receptor gene
CT Noguchi, KS Bae, K Chin, Y Wada, AN Schechter and WD Hankins
Laboratory of Chemical Biology, NIDDK, National Institutes of Health,
Bethesda, MD 20892.
We have isolated and characterized a genomic clone of the human
erythropoietin (Epo) receptor from a placental genomic library using a cDNA
probe for the murine Epo receptor. The coding region spans about 6.5 kb
with seven intervening sequences ranging in size from 81 bp to 2.1 kb. A
stretch of 123 purines is found in the 5' region from -456 to -578 upstream
from the first codon and flanking the Alu repetitive sequences located
further upstream. The human Epo receptor contains a palindromic sequence 5'
of the translated region that consists of an almost perfect inverted repeat
of 12 nucleotides (CAGCTGC(G/C)TCCG) centered about G at -92 from the first
codon. An inverted SP1 binding site (CCGCCC) and an inverted GATA-1 binding
site (TTATCT) are located at positions -151 and -179, respectively, and
CACCC sequences are located at -585 and further upstream. No TATA or CAAT
sequences are in this 5' flanking region. However, this region as far as
-275 has a 72% GC content compared with an overall GC content of 56%. A
1-kb BamHI fragment of the human Epo receptor containing 700 bp of
sequences 5' of the coding region was transcribed in an in vitro
transcription assay; initiation of transcription appeared to be around 132
+/- 5 just downstream from the inverted SP1 site at -151. T1 analysis of
human Epo receptor messenger RNA also maps the site of transcription
initiation to this region. Within 180 nucleotides 5' to the first exon are
three regions with 70% or greater homology with the murine Epo receptor.
The study of this gene, including its similarities with the murine Epo
receptor, should help elucidate aspects of the transcriptional and possible
translational control of the Epo receptor in human erythroid cells and thus
its role in signal transduction and erythroid differentiation.
Volume 78,
Issue 10,
pp. 2548-2556,
11/15/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. D. Semba and S. E. Juul
Erythropoietin in Human Milk: Physiology and Role in Infant Health
J Hum Lact,
August 1, 2002;
18(3):
252 - 261.
[Abstract]
[PDF]
|
|
|
|
|
|
|
V. Divoky, J. T. Prchal ;, X. Yu, and C. T. Noguchi
Mouse surviving solely on human erythropoietin receptor (EpoR): model of human EpoR-linked disease
Blood,
May 15, 2002;
99(10):
3873 - 3875.
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Yu, C.-S. Lin, F. Costantini, and C. T. Noguchi
The human erythropoietin receptor gene rescues erythropoiesis and developmental defects in the erythropoietin receptor null mouse
Blood,
July 15, 2001;
98(2):
475 - 477.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Fujio, T. Nosaka, T. Kojima, T. Kawashima, T. Yahata, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, K. Yamamoto, T. Nishimura, et al.
Molecular cloning of a novel type 1 cytokine receptor similar to the common gamma chain
Blood,
April 1, 2000;
95(7):
2204 - 2210.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kafert, S. Luther, I. Boll, K. Wagner, A. Ganser, and M. Eder
Functional Analysis of a Single Chain Chimeric alpha /beta -Granulocyte-Macrophage Colony-stimulating Factor Receptor. IMPORTANCE OF A GLUTAMATE RESIDUE IN THE TRANSMEMBRANE REGION
J. Biol. Chem.,
November 12, 1999;
274(46):
33064 - 33071.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Miller, D. L. Barber, L. L. Bell, B. K. Beattie, M.-Y. Zhang, B. G. Neel, M. Yoakim, L. I. Rothblum, and J. Y. Cheung
Identification of the Erythropoietin Receptor Domain Required for Calcium Channel Activation
J. Biol. Chem.,
July 16, 1999;
274(29):
20465 - 20472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Sato, S. Watanabe, E. Ishii, K. Tsuji, and T. Nakahata
Induction of the Erythropoietin Receptor Gene and Acquisition of Responsiveness to Erythropoietin by Stem Cell Factor in HML/SE, a Human Leukemic Cell Line
J. Biol. Chem.,
July 3, 1998;
273(27):
16921 - 16926.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Liu, K. Shen, Z. Liu, and C. T. Noguchi
Regulated Human Erythropoietin Receptor Expression in Mouse Brain
J. Biol. Chem.,
December 19, 1997;
272(51):
32395 - 32400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Kralovics, K. Indrak, T. Stopka, B. W. Berman, J. F. Prchal, and J. T. Prchal
Two New EPO Receptor Mutations: Truncated EPO Receptors Are Most Frequently Associated With Primary Familial and Congenital Polycythemias
Blood,
September 1, 1997;
90(5):
2057 - 2061.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Chiba, T. Takahashi, K. Takeshita, J. Minowada, Y. Yazaki, F. H. Ruddle, and H. Hirai
Selective Expression of mRNA Coding for the Truncated Form of Erythropoietin Receptor in Hematopoietic Cells and Its Decrease in Patients With Polycythemia Vera
Blood,
July 1, 1997;
90(1):
97 - 104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. O. Arcasoy, B. A. Degar, K. W. Harris, and B. G. Forget
Familial Erythrocytosis Associated With a Short Deletion in the Erythropoietin Receptor Gene
Blood,
June 15, 1997;
89(12):
4628 - 4635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Y. Cheung, X.-Q. Zhang, K. Bokvist, D. L. Tillotson, and B. A. Miller
Modulation of Calcium Channels in Human Erythroblasts by Erythropoietin
Blood,
January 1, 1997;
89(1):
92 - 100.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Nishikawa, M. Oishi, and R. Kiyama
Construction of a Human Genomic Library of Clones Containing Poly(dG-dA)[IMAGE]Poly(dT-dC) Tracts by Mg[IMAGE]-dependent Triplex Affinity Capture
J. Biol. Chem.,
April 21, 1995;
270(16):
9258 - 9264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. A. Tabbara
Erythropoietin: Biology and Clinical Applications
Arch Intern Med,
February 8, 1993;
153(3):
298 - 304.
[Abstract]
[PDF]
|
|
|
|
|
|
|
Y. Nakamura, N. Komatsu, and H. Nakauchi
A Truncated Erythropoietin Receptor That Fails to Prevent Programmed Cell Death of Erythroid Cells
Science,
August 21, 1992;
257(5073):
1138 - 1141.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Ogilvie, X. Yu, V. Nicolas-Metral, S. M. Pulido, C. Liu, U. T. Ruegg, and C. T. Noguchi
Erythropoietin Stimulates Proliferation and Interferes with Differentiation of Myoblasts
J. Biol. Chem.,
December 8, 2000;
275(50):
39754 - 39761.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
