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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kan, O. Articles by Whetton, A. D. Search for Related Content PubMed PubMed Citation Articles by Kan, O. Articles by Whetton, A. D. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Interferon-gamma stimulates the survival and influences the development of bipotential granulocyte-macrophage colony-forming cells O Kan, CM Heyworth, TM Dexter, PJ Maudsley, N Cook, SJ Vallance and AD Whetton Department of Biochemistry and Applied Molecular Biology, UMIST, Manchester, UK. The effects of interferon-gamma (IFN-gamma) on a highly enriched population of granulocyte-macrophage colony-forming cells (GM-CFC) were assessed. When added with myeloid growth factors (interleukin-3 [IL-3], granulocyte-macrophage colony-stimulating factor [GM-CSF], or macrophage-CSF [M-CSF]), IFN-gamma inhibited the formation of colonies in soft agar assays. Furthermore IFN-gamma stimulated an increase in the number of macrophages present in colonies formed in the presence of IL-3. IFN-gamma also inhibited M-CSF-, GM-CSF-, or IL-3-stimulated [3H]- thymidine incorporation in highly enriched GM-CFC. However, when added in the absence of hematopoietic growth factors, IFN-gamma promoted the survival of GM-CFC and had a modest stimulatory effect on DNA synthesis. The direct interaction of the IFN with GM-CFC was confirmed by showing its ability to rapidly activate the sodium/hydrogen antiport in GM-CFC, as do the mitogens GM-CSF, M-CSF, and IL-3. However, the effect of IFN-gamma on intracellular pH and DNA synthesis was transient and pretreatment with IFN markedly inhibited the ability of GM-CSF, M- CSF, and IL-3 to activate the sodium/hydrogen antiport. IFN-gamma has a dual effect on GM-CFC, decreasing the rate of cell death but also limiting the proliferative response to CSFs. Volume 78, Issue 10, pp. 2588-2594, 11/15/1991 Copyright © 1991 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Pierce, C. M. Heyworth, S. E. Nicholls, E. Spooncer, T. M. Dexter, J. M. Lord, P. J. Owen-Lynch, G. Wark, and A. D. Whetton An Activated Protein Kinase C {alpha} Gives a Differentiation Signal for Hematopoietic Progenitor Cells and Mimicks Macrophage Colony-stimulating Factor-stimulated Signaling Events J. Cell Biol., March 23, 1998; 140(6): 1511 - 1518. [Abstract] [Full Text] [PDF]

	Copyright © 1991 by American Society of Hematology         Online ISSN: 1528-0020