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Lambda-like and V pre-B genes expression: an early B-lineage marker of
human leukemias
C Schiff, M Milili, D Bossy, A Tabilio, F Falzetti, J Gabert, P Mannoni and M Fougereau
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France.
V pre-B and lambda-like genes are selectively expressed in human pre-B
cells and encode polypeptide chains that associate in a mu-pseudolight
chain complex that may regulate some crucial steps of early B-cell
differentiation. We have followed by polymerase chain reaction and Northern
blot analysis the expression of these "pre-B-specific" genes in correlation
with the status (rearranged v germline) of Ig gene loci (H, kappa, lambda)
in a panel of 32 leukemias pertaining mostly to the B lineage and including
a number of ambiguously characterized samples. All cells that had
rearranged the H locus only expressed V pre-B and lambda-like transcripts,
in agreement with a pre-B status. In this group, some biphenotypic
leukemias (mostly My/B) might, in fact, be already engaged in the B
lineage. Rearrangement of V kappa or V lambda loci correlated with the
disappearance of the pre-B gene products. In a pre-B acute lymphoblastic
leukemia cell line that was induced to mature to the B-cell stage in
culture upon kappa gene rearrangement, the mu- pseudolight chain complex
was actually replaced by the classical mu- kappa molecule. Finally, V pre-B
and lambda-like genes were found expressed in two leukemic cells that had
retained all Ig loci in germline configuration. This finding raises the
possibility of having an early pro-B progenitor in which V pre-B and
lambda-like products associate with a H chain surrogate in a complex that
would trigger an early event of B-cell differentiation such as the H locus
rearrangements.
Volume 78,
Issue 6,
pp. 1516-1525,
09/15/1991
Copyright © 1991 by The American Society of Hematology
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