Factor-dependent erythroid cell lines derived from mice transplanted with
hematopoietic cells expressing the v-src oncogene
M Kennedy, H Beug, EF Wagner and G Keller
National Jewish Center for Immunology and Respiratory Medicine, Denver, CO
80206.
Transplantation of spleen cells from primary reconstituted mice expressing
the v-src oncogene to secondary and tertiary irradiated recipients resulted
in the emergence of erythroid precursors with a transformed phenotype. When
cultured in methyl cellulose, these precursors generated colonies of
undifferentiated cells that could be expanded into continuously growing
factor-dependent cell lines in liquid culture. All lines tested had a
similar phenotype and expressed the v-src oncogene. In addition they
responded to factors that regulate normal erythroid development, namely
erythropoietin (Epo), interleukin- 3 (IL-3), and mast cell growth factor
(MGF), the ligand to the c-kit encoded receptor. When cells from one of the
lines were maintained in the absence of factor, a "factor independent"
subpopulation emerged that appeared to grow in an autocrine fashion.
Conditioned medium from these cells stimulated their own growth as well as
the growth of broad spectrum of normal precursors. Studies with
neutralizing antibodies indicated that the predominant colony-stimulating
factor produced by these cells is IL-3.
Volume 79,
Issue 1,
pp. 180-190,
01/01/1992
Copyright © 1992 by The American Society of Hematology
