|
|
 Previous Article | Table of Contents | Next Article 
Cytokine regulation of colony-stimulating factor (CSF) production in
cultured human synovial fibroblasts. II. Similarities and differences in
the control of interleukin-1 induction of granulocyte-macrophage CSF and
granulocyte-CSF production
JA Hamilton, DS Piccoli, J Cebon, JE Layton, P Rathanaswani, SR McColl and T Leizer
Department of Medicine, University of Melbourne, Royal Melbourne Hospital,
Parkville, Australia.
Synovial fibroblasts are likely to be a significant source of
granulocyte-macrophage colony-stimulating factor (GM-CSF) and
granulocyte-CSF (G-CSF), which could be crucial to the pathogenesis of
rheumatoid arthritis. Using specific enzyme-linked immunosorbent assays
(ELISAs) and Northern analysis, GM-CSF and G-CSF expression were followed
in human synovial fibroblast-like cells in response to a number of agents,
either alone or in the presence of an optimal stimulatory concentration of
interleukin-1 (IL-1). For both CSFs, interferon-gamma (100 U/mL) did not
increase their levels but dramatically suppressed the stimulatory action of
IL-1, while basic fibroblast growth factor (10(-8) mol/L), although
nonstimulatory by itself, potentiated IL-1 action. The glucocorticoid,
dexamethasone (10(- 7) mol/L), inhibited IL-1-stimulated CSF production.
However, evidence was obtained for noncoordinated CSF regulation.
Cyclooxygenase inhibitors potentiated the action of IL-1 on GM-CSF
synthesis but suppressed G-CSF synthesis, suggesting that endogenous
cyclooxygenase products can have opposite effects in modulating the levels
of each CSF. Also, the lymphokine, IL-4 (250 pmol/L), slightly inhibited
GM-CSF formation in the presence of IL-1 but elevated the G-CSF levels in
these cultures without having an effect by itself. Transforming growth
factor beta (less than or equal to 20 ng/mL) did not modulate levels of
either CSF. Mesenchymal cell production of both GM-CSF and G-CSF is
generally viewed as being under coordinate control; our findings suggest
that their synthesis in IL-1-stimulated human synoviocytes can be modulated
by a number of agents, in some cases with divergent actions depending on
which CSF is examined.
Volume 79,
Issue 6,
pp. 1413-1419,
03/15/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Del Rosso, M. Cinelli, S. Guiducci, A. Pignone, G. Fibbi, F. Margheri, A. Gabrielli, R. Giacomelli, A. Coppini, M. Del Rosso, et al.
Deflazacort modulates the fibrinolytic pattern and reduces uPA-dependent chemioinvasion and proliferation in rheumatoid arthritis synoviocytes
Rheumatology,
October 1, 2005;
44(10):
1255 - 1262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. L. Wamsley, U. T. Iwaniec, and T. J. Wronski
Selected Extraskeletal Effects of Systemic Treatment with Basic Fibroblast Growth Factor in Ovariectomized Rats
Toxicol Pathol,
August 1, 2005;
33(5):
577 - 583.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. H. Qureshi, K. M. Empey, and B. A. Garvy
Modulation of Proinflammatory Responses to Pneumocystis carinii f. sp. muris in Neonatal Mice by Granulocyte-Macrophage Colony-Stimulating Factor and IL-4: Role of APCs
J. Immunol.,
January 1, 2005;
174(1):
441 - 448.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C M Gelbmann, S N Leeb, D Vogl, M Maendel, H Herfarth, J Scholmerich, W Falk, and G Rogler
Inducible CD40 expression mediates NF{kappa}B activation and cytokine secretion in human colonic fibroblasts
Gut,
October 1, 2003;
52(10):
1448 - 1456.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Mueller, C. C. Herold-Mende, D. Riede, M. Lange, H.-H. Steiner, and N. E. Fusenig
Autocrine Growth Regulation by Granulocyte Colony-Stimulating Factor and Granulocyte Macrophage Colony-Stimulating Factor in Human Gliomas with Tumor Progression
Am. J. Pathol.,
November 1, 1999;
155(5):
1557 - 1567.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Pang and A. J. Knox
Bradykinin Stimulates IL-8 Production in Cultured Human Airway Smooth Muscle Cells: Role of Cyclooxygenase Products
J. Immunol.,
September 1, 1998;
161(5):
2509 - 2515.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
