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Correlation of CD2 expression with PML gene breakpoints in patients with
acute promyelocytic leukemia [see comments]
DF Claxton, CL Reading, L Nagarajan, Y Tsujimoto, BS Andersson, E Estey, A Cork, YO Huh, J Trujillo and AB Deisseroth
Department of Hematology, University of Texas M.D. Anderson Cancer Center,
Houston 77030.
The chromosomal translocation t(15;17)(q22:21) of acute promyelocytic
leukemia (APL) fuses PML, a novel gene, with RAR alpha, a retinoic acid
receptor gene. PML-RAR hybrid transcripts were studied in 18 cases of APL
using RNA-PCR. Two forms were noted: one designated 5', producing a 439-bp
chimeric fragment, and a 3' form, producing a pair of fragments of 765 bp
and 909 bp. 5' forms were found in 7 of the 18 cases while the other 11
patients expressed the 3' forms. The chromosome 15 specific probes K3 and
K2 were used to study genomic breakpoints in 12 APL patients. Comparison of
these results with RNA PCR in 11 patients for whom both were available
yielded a rearrangement pattern predictive of whether the hybrid transcript
was 5' or 3'. In this way, an additional three patients in whom DNA but not
RNA was available were identified as having 3' (downstream) breakpoints
and, therefore, 3' hybrid forms. Thus, 21 cases categorized as having 5' or
3' PML-RAR transcripts were analyzed for various phenotypic differences.
Surface phenotyping of leukemic promyelocytes demonstrated expression of
the CD2 antigen in all cases with the 5' splice variant. Only 1 of 11 cases
with the 3' form showed CD2 expression. This difference is significant at P
= .001.
Volume 80,
Issue 3,
pp. 582-586,
08/01/1992
Copyright © 1992 by The American Society of Hematology
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